Overview

  • Product nameAnti-EAAT1 antibody
    See all EAAT1 primary antibodies
  • Description
    Rabbit polyclonal to EAAT1
  • SpecificityThis antibody reacts with Glutamate-aspartate transporter in Rat CNS samples.
  • Tested applicationsSuitable for: IHC-FoFr, WB, ICC, Flow Cyt, IHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to 20 residues from the C-terminus of rat EAAT1 (Peptide available as ab127026.)

  • General notesGlutamate-aspartate transporter is also known as GLAST.

Properties

Applications

Our Abpromise guarantee covers the use of ab416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 18714338
WB 1/200 - 1/5000. Predicted molecular weight: 60 kDa.Can be blocked with EAAT1 peptide (ab127026).
ICC Use at an assay dependent concentration.
Flow Cyt 1/10 - 1/200.
IHC-P 1/50 - 1/500.
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/400.

Target

  • FunctionTransports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
  • Tissue specificityHighly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
  • Involvement in diseaseDefects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia.
  • Sequence similaritiesBelongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EA6 antibody
    • EAA1_HUMAN antibody
    • EAAT1 antibody
    • Excitatory amino acid transporter 1 antibody
    • FLJ25094 antibody
    • GLAST antibody
    • GLAST-1 antibody
    • GLAST1 antibody
    • Glial high affinity glutamate transporter antibody
    • glutamate/aspartate transporter, high affinity, sodium-dependent antibody
    • High affinity neuronal glutamate transporter antibody
    • Slc1a3 antibody
    • Sodium dependent glutamate/aspartate transporter antibody
    • Sodium-dependent glutamate/aspartate transporter 1 antibody
    • Solute carrier family 1 (glial high affinity glutamate transporter) member 3 antibody
    • Solute carrier family 1 member 3 antibody
    see all

Anti-EAAT1 antibody images

  • ab416 staining zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5%BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab416 at 1/100 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. EAAT1 expression can be observed in the inner plexiform layer (in green).

    See Abreview

  • ab416 staining EAAT1 in Chicken retina cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.02% in PBS and blocked with 5% serum for 16 hours at 22°C. Samples were incubated with primary antibody (1/500 in PBS + 0.02% Triton) for 16 hours at 22°C. An undiluted Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Anti-EAAT1 antibody (ab416) + Rat brain cortex

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 150 kDa (possible dimer).
  • ab416 at 1/100 dilution staining EAAT1 in mouse coronal tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Adult Sprague–Dawley rats were injected intraperitoneally with pentobarbitone and were transcardially perfused-fixed with heparinized saline and 4% paraformaldehyde in PBS. Brains were removed, were postfixed in 4% paraformaldehyde for 5h, and were stored in PBS. Sections of each brain were pretreated with citrate buffer for 30 min at 65°C to increase antigen retrieval and penetration of the antibodies into the tissues. Sections were permeabilized with 1% Triton X-100 for 5 min and blocked with 3% normal horse serum in 0.1 M PBS, pH 7.4, for 60 min and incubated at room temperature for 48h with primary antibodies. An Alexa Fluor® 488 conjugated anti rabbit was used as secondary at 1/1000 dilution.

  • ab416 (1:500) staining EAAT1 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of membrane cells in the purkinje glial region .
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ab416 staining EAAT1 in Rat Astrocyte cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% Serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal(1/300) was used as the secondary antibody.

    See Abreview

References for Anti-EAAT1 antibody (ab416)

This product has been referenced in:
  • Vong KI  et al. Sox9 is critical for suppression of neurogenesis but not initiation of gliogenesis in the cerebellum. Mol Brain 8:25 (2015). Read more (PubMed: 25888505) »
  • Sohn J  et al. The subventricular zone continues to generate corpus callosum and rostral migratory stream astroglia in normal adult mice. J Neurosci 35:3756-63 (2015). IHC-FoFr ; Mouse . Read more (PubMed: 25740506) »

See all 27 Publications for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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My sincere apologies for the delay, I have been trying to find out the epitope for ab416 and was unfortunately unsuccessful. The glutamate aspartate transporter GLAST is actually located both in the cytoplasm and at the membrane: it can be found lo...

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Application Flow Cytometry
Sample Human Cell (Differentiated hNSCs)
Permeabilization Yes - 0.25% Triton X-100 in DPBS
Gating Strategy Undifferentiated Stem Cells (white)
Specification Differentiated hNSCs
Preparation Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 09 2015

Application Western blot
Sample Rabbit Tissue lysate - whole (brain)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification brain
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 23 2015

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (Retina, Inner plexiform layer)
Specification Retina, Inner plexiform layer
Permeabilization Yes - 0.1% TritonX
Fixative Paraformaldehyde
Username

Dr. Ryan MacDonald

Verified customer

Submitted Jun 05 2013

Application Immunocytochemistry/ Immunofluorescence
Sample Chicken Cell (retina)
Specification retina
Fixative Paraformaldehyde
Permeabilization Yes - triton X-100 (0.02%) diluted in PBS
Blocking step Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Chris Zelinka

Verified customer

Submitted Apr 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Astrocytes)
Specification Astrocytes
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Astrocytes)
Loading amount 30 µg
Specification Astrocytes
Gel Running Conditions Reduced Denaturing (12)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (brain)
Specification brain
Fixative Formaldehyde
Permeabilization Yes - 0.02%-Triton
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 24 2013

Thank you for your enquiry.

I can confirm that the immunogen for ab416 EAAT1 antibody, the immunogen is located within the last 100 amino acids of the protein. According to the UniProt protein database page for this protein, this would be in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"