Recombinant
RabMAb

Anti-EAAT1 antibody [EPR12686] (ab181036)

Overview

  • Product name
    Anti-EAAT1 antibody [EPR12686]
    See all EAAT1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12686] to EAAT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFr, IHC-P, WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human EAAT1 aa 150-250. The exact sequence is proprietary.
    Database link: P43003

  • Positive control
    • Mouse brain, rat brain and human cerebellum lysates. Human brain tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/50 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
    • Tissue specificity
      Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
    • Involvement in disease
      Defects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia.
    • Sequence similarities
      Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily.
    • Post-translational
      modifications
      Glycosylated.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • EA6 antibody
      • EAA1_HUMAN antibody
      • EAAT1 antibody
      • Excitatory amino acid transporter 1 antibody
      • FLJ25094 antibody
      • GLAST antibody
      • GLAST-1 antibody
      • GLAST1 antibody
      • Glial high affinity glutamate transporter antibody
      • glutamate/aspartate transporter, high affinity, sodium-dependent antibody
      • High affinity neuronal glutamate transporter antibody
      • Slc1a3 antibody
      • Sodium dependent glutamate/aspartate transporter antibody
      • Sodium-dependent glutamate/aspartate transporter 1 antibody
      • Solute carrier family 1 (glial high affinity glutamate transporter) member 3 antibody
      • Solute carrier family 1 member 3 antibody
      see all

    Images

    • ab181036 staining EAAT1 in rat cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

    • ab181036 staining EAAT1 in mouse cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

      Negative control 1: PBS in place of primary antibody.

    • ab181036 staining EAAT1 in human glioma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

      Negative control 1: PBS in place of primary antibody.

    • ab181036 staining EAAT1 in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

      Negative control 1: PBS in place of primary antibody.

    • All lanes : Anti-EAAT1 antibody [EPR12686] (ab181036) at 1/5000 dilution

      Lane 1 : Mouse brain
      Lane 2 : Mouse hippocampus
      Lane 3 : Rat hippocampus
      Lane 4 : Rat brain

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 59 kDa
      Additional bands at: 59 kDa. We are unsure as to the identity of these extra bands.

    • Anti-EAAT1 antibody [EPR12686] (ab181036) at 1/5000 dilution + Human cerebellum at 20 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 59 kDa
      Additional bands at: 59 kDa. We are unsure as to the identity of these extra bands.



      Blocking and diluting buffer: 5% NFDM/TBST

      The band above (100KDa) is dimer

    • ab181036 staining EAAT1 in monkey brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, blocked with 10% serum at 24°C and antigen retrieval was by heat mediation in 10mM citrate acid + 0.05% Tween 20. The sample was incubated with primary antibody (1/100 in 2% donkey buffer) at 24°C for 13 hours. An Alexa Fluor® 647-conjugated donkey anti-rabbit polyclonal (1/100) was used as the secondary antibody.

      See Abreview

    • All lanes : Anti-EAAT1 antibody [EPR12686] (ab181036) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Rat brain lysate
      Lane 3 : Human cerebellum lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 59 kDa

    • Immunohistochemical staining of EAAT1 in paraffin-embedded human brain tissue using ab181036 at a 1/50 dilution.

    References

    This product has been referenced in:
    • Wang R  et al. Astrocytic JWA deletion exacerbates dopaminergic neurodegeneration by decreasing glutamate transporters in mice. Cell Death Dis 9:352 (2018). Read more (PubMed: 29500411) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citric acid 0.05% Tween20
    Sample
    Monkey Tissue sections (brain)
    Specification
    brain
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 27 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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