Anti-EAAT2 antibody (ab41621)
Key features and details
- Rabbit polyclonal to EAAT2
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-EAAT2 antibody
See all EAAT2 primary antibodies -
Description
Rabbit polyclonal to EAAT2 -
Host species
Rabbit -
Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide within Rat EAAT2 aa 550 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P31596 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 10 µg/ml.
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
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Notes |
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ICC
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). |
Target
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Function
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. -
Sequence similarities
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 20511 Mouse
- Entrez Gene: 29482 Rat
- SwissProt: P43006 Mouse
- SwissProt: P31596 Rat
- Unigene: 267547 Mouse
- Unigene: 371582 Mouse
- Unigene: 10240 Rat
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Alternative names
- EAA2_HUMAN antibody
- EAAT2 antibody
- Excitatory amino acid transporter 2 antibody
see all
Images
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ab41621 staining EAAT2 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab41621 at 10µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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All lanes : Anti-EAAT2 antibody (ab41621) at 1 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 62 kDa -
Methanol fixed human astrocytes (differentiated from H9-derived neuronal) cells labeling EAAT2 using ab41621 at a 1/250 dilution, 1 hr, 22°C (green) followed by an Alexa Fluor® 488 secondary antibody (1/1000 dilution), in ICC/IF.
Cells were blocked in 5% BSA for 30 mins, 22°C.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (76)
ab41621 has been referenced in 76 publications.
- Schwenke KA et al. Primary glia cells from bank vole propagate multiple rodent-adapted scrapie prions. Sci Rep 12:2190 (2022). PubMed: 35140295
- Kabdesh IM et al. Cellular and Molecular Gradients in the Ventral Horns With Increasing Distance From the Injury Site After Spinal Cord Contusion. Front Cell Neurosci 16:817752 (2022). PubMed: 35221924
- Shah D et al. Astrocyte calcium dysfunction causes early network hyperactivity in Alzheimer's disease. Cell Rep 40:111280 (2022). PubMed: 36001964
- Alhaddad H et al. Effects of a Novel Beta Lactam Compound, MC-100093, on the Expression of Glutamate Transporters/Receptors and Ethanol Drinking Behavior of Alcohol-Preferring Rats. J Pharmacol Exp Ther 383:208-216 (2022). PubMed: 36153003
- Wang JY et al. Electroacupuncture relieves hyperalgesia by regulating neuronal-glial interaction and glutamate transporters of spinal dorsal horns in rats with acute incisional neck pain. Front Neurosci 16:885107 (2022). PubMed: 36389227