This antibody gave a positive signal in the following Mouse tissue lysates: Brain; Cerebellum; Cortex.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). Abcam recommends using milk as the blocking agent - 3%
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.
solute carrier family 1 (glial high affinity glutamate transporter), member 2 antibody
Solute carrier family 1 glial high affinity glutamate transporter member 2 antibody
Solute carrier family 1 member 2 antibody
Western blot - Anti-EAAT2 antibody (ab94667)
All lanes : Anti-EAAT2 antibody (ab94667) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Brain (Mouse) Tissue Lysate Lane 2 : Cerebellum Mouse Tissue Lysate Lane 3 : Mouse Cortex Tissue Lysate
Lysates/proteins at 25 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa Observed band size: 62 kDa Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab94667 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.