The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000.
1/100 - 1/250.
1/10000 - 1/50000.
Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).
Is unsuitable for IP.
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5.
Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers).
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A1 subfamily.
Flow cytometry analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labeling EAAT3 (red) with ab124802 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)
All lanes : Anti-EAAT3 antibody [EPR6774(B)] (ab124802) at 1/10000 dilution
Lane 1 : Mouse brain tissue lysate Lane 2 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Ramos-Chávez LA et al. Neurological effects of inorganic arsenic exposure: altered cysteine/glutamate transport, NMDA expression and spatial memory impairment. Front Cell Neurosci9:21 (2015).
Read more (PubMed: 25709567) »
Martinez-Lozada Z et al. Activation of sodium-dependent glutamate transporters regulates the morphological aspects of oligodendrocyte maturation via signaling through calcium/calmodulin-dependent kinase IIß's actin-binding/-stabilizing domain. Glia62:1543-58 (2014).
Read more (PubMed: 24866099) »