The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000.
1/100 - 1/250.
1/10000 - 1/50000.
Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).
Application notesIs unsuitable for IP.
FunctionTransports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5.
Tissue specificityExpressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers).
Sequence similaritiesBelongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A1 subfamily.
References for Anti-EAAT3 antibody [EPR6774(B)] (ab124802)
This product has been referenced in:
Martinez-Lozada Z et al. Activation of sodium-dependent glutamate transporters regulates the morphological aspects of oligodendrocyte maturation via signaling through calcium/calmodulin-dependent kinase IIß's actin-binding/-stabilizing domain. Glia62:1543-58 (2014).
Read more (PubMed: 24866099) »