Overview

  • Product nameAnti-EAAT3 antibody [EPR6774(B)]
    See all EAAT3 primary antibodies
  • Description
    Rabbit monoclonal [EPR6774(B)] to EAAT3
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • Human fetal brain and human testis tissue lysate; Human kidney tissue; Mouse and rat brain tissue lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-EAAT3 antibody (HRP) [EPR6774(B)] (ab203479)

Properties

Applications

Our Abpromise guarantee covers the use of ab124802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000.
ICC/IF 1/100 - 1/250.
WB 1/10000 - 1/50000.

Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionTransports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5.
    • Tissue specificityExpressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers).
    • Sequence similaritiesBelongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A1 subfamily.
    • Post-translational
      modifications
      Glycosylated.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • EAA3_HUMAN antibody
      • EAAC 1 antibody
      • EAAC1 antibody
      • EAAT 3 antibody
      • Excitatory amino acid carrier 1 antibody
      • Excitatory amino acid carrier1 antibody
      • Excitatory amino acid transporter 3 antibody
      • Excitatory amino acid transporter3 antibody
      • Excitatory amino-acid carrier 1 antibody
      • GLUTAMATE TRANSPORTER, HIGH-AFFINITY antibody
      • MEAAC 1 antibody
      • MEAAC1 antibody
      • Neuronal and epithelial glutamate transporter antibody
      • REAAC 1 antibody
      • REAAC1 antibody
      • Slc1 a1 antibody
      • Slc1a 1 antibody
      • SLC1A1 antibody
      • Sodium dependent glutamate/aspartate transporter 3 antibody
      • Sodium-dependent glutamate/aspartate transporter 3 antibody
      • Solute carrier family 1 (neuronal / epithelial high affinity glutamate transporter, system Xag), member 1 antibody
      • SOLUTE CARRIER FAMILY 1 (NEURONAL/EPITHELIAL HIGH AFFINITY GLUTAMATE TRANSPORTER), MEMBER 1 antibody
      • Solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 antibody
      • Solute carrier family 1 member 1 antibody
      • Solute carrier family 1, member 1 antibody
      see all

    Anti-EAAT3 antibody [EPR6774(B)] images

    • All lanes : Anti-EAAT3 antibody [EPR6774(B)] (ab124802) at 1/50000 dilution

      Lane 1 : Human fetal brain tissue lysate
      Lane 2 : Human testis tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Observed band size : 70 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

      Blocking and Diluting buffer : 5% NFDM/TBST

    • ab124802, at 1/500 dilution, staining EAAT3 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • All lanes : Anti-EAAT3 antibody [EPR6774(B)] (ab124802) at 1/10000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Observed band size : 70 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      Blocking and Diluting buffer : 5% NFDM/TBST

      An additional band detected at 100 kDa is unidentified.

       

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-EAAT3 antibody [EPR6774(B)] (ab124802)

    This product has been referenced in:
    • Martinez-Lozada Z  et al. Activation of sodium-dependent glutamate transporters regulates the morphological aspects of oligodendrocyte maturation via signaling through calcium/calmodulin-dependent kinase IIß's actin-binding/-stabilizing domain. Glia 62:1543-58 (2014). Read more (PubMed: 24866099) »

    See 1 Publication for this product

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"