Recombinant
RabMAb

Anti-EAAT3 antibody [EPR6774(B)] (ab124802)

Overview

  • Product name
    Anti-EAAT3 antibody [EPR6774(B)]
    See all EAAT3 primary antibodies
  • Description
    Rabbit monoclonal [EPR6774(B)] to EAAT3
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, ICC/IF, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human EAAT3 aa 150-250. The exact sequence is proprietary.

  • Positive control
    • WB: Human fetal brain, human testis, mouse brain and rat brain tissue lysates. IHC-P: Human kidney tissue. Flow Cyt: U-87 MG cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200.
IHC-P 1/500 - 1/1000.
ICC/IF 1/100 - 1/250.
WB 1/10000 - 1/50000.

Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5.
    • Tissue specificity
      Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers).
    • Sequence similarities
      Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A1 subfamily.
    • Post-translational
      modifications
      Glycosylated.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • EAA3_HUMAN antibody
      • EAAC 1 antibody
      • EAAC1 antibody
      • EAAT 3 antibody
      • Excitatory amino acid carrier 1 antibody
      • Excitatory amino acid carrier1 antibody
      • Excitatory amino acid transporter 3 antibody
      • Excitatory amino acid transporter3 antibody
      • Excitatory amino-acid carrier 1 antibody
      • GLUTAMATE TRANSPORTER, HIGH-AFFINITY antibody
      • MEAAC 1 antibody
      • MEAAC1 antibody
      • Neuronal and epithelial glutamate transporter antibody
      • REAAC 1 antibody
      • REAAC1 antibody
      • Slc1 a1 antibody
      • Slc1a 1 antibody
      • SLC1A1 antibody
      • Sodium dependent glutamate/aspartate transporter 3 antibody
      • Sodium-dependent glutamate/aspartate transporter 3 antibody
      • Solute carrier family 1 (neuronal / epithelial high affinity glutamate transporter, system Xag), member 1 antibody
      • SOLUTE CARRIER FAMILY 1 (NEURONAL/EPITHELIAL HIGH AFFINITY GLUTAMATE TRANSPORTER), MEMBER 1 antibody
      • Solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 antibody
      • Solute carrier family 1 member 1 antibody
      • Solute carrier family 1, member 1 antibody
      see all

    Anti-EAAT3 antibody [EPR6774(B)] images

    • All lanes : Anti-EAAT3 antibody [EPR6774(B)] (ab124802) at 1/50000 dilution

      Lane 1 : Human fetal brain tissue lysate
      Lane 2 : Human testis tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Observed band size : 70 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

      Blocking and Diluting buffer : 5% NFDM/TBST

    • ab124802, at 1/500 dilution, staining EAAT3 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • Flow cytometry analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labeling EAAT3 (red) with ab124802 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

    • All lanes : Anti-EAAT3 antibody [EPR6774(B)] (ab124802) at 1/10000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Observed band size : 70 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      Blocking and Diluting buffer : 5% NFDM/TBST

      An additional band detected at 100 kDa is unidentified.

       

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-EAAT3 antibody [EPR6774(B)] (ab124802)

    This product has been referenced in:
    • Ramos-Chávez LA  et al. Neurological effects of inorganic arsenic exposure: altered cysteine/glutamate transport, NMDA expression and spatial memory impairment. Front Cell Neurosci 9:21 (2015). WB ; Mouse . Read more (PubMed: 25709567) »
    • Martinez-Lozada Z  et al. Activation of sodium-dependent glutamate transporters regulates the morphological aspects of oligodendrocyte maturation via signaling through calcium/calmodulin-dependent kinase IIß's actin-binding/-stabilizing domain. Glia 62:1543-58 (2014). Read more (PubMed: 24866099) »

    See all 2 Publications for this product

    Product Wall

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human umbilical vein endothelial cell)
    Gel Running Conditions
    Non-reduced Denaturing (10% gel)
    Loading amount
    20 µg
    Specification
    Human umbilical vein endothelial cell
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 01 2017

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Brain)
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 12 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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