Overview

  • Product name
  • Description
    Rabbit polyclonal to EAAT4
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Dog, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human EAAT4.

    (Peptide available as ab45367.)

  • Positive control
    • This antibody gave a positive signal in Mouse Cerebellum tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab41650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 mg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 61 kDa).

Target

  • Relevance
    EAAT4 is expressed predominantly in the cerebellum (Purkinje cells) and weakly in the forebrain, where it transports L-glutamate, L-aspartate and D-aspartate, acting as a symport by cotransporting sodium. EAAT4 may function as a combined transporter and inhibitory glutamate receptor and also functions as an anion-selective ion channel.
  • Cellular localization
    Membrane; multi-pass membrane protein.
  • Database links
  • Alternative names
    • Excitatory amino acid transporter 4 antibody
    • High affinity neuronal glutamate transporter antibody
    • MGC33092 antibody
    • MGC43671 antibody
    • Sodium dependent glutamate/aspartate transporter antibody
    • Solute carrier family 1 (high affinity aspartate/glutamate transporter) member 6 antibody
    • Solute carrier family 1 member 6 antibody
    see all

Images

  • Anti-EAAT4 antibody (ab41650) at 1 µg/ml + Cerebellum Mouse Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 66 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References

This product has been referenced in:
  • Ramachandran PS  et al. Nonallele Specific Silencing of Ataxin-7 Improves Disease Phenotypes in a Mouse Model of SCA7. Mol Ther N/A:N/A (2014). Read more (PubMed: 24930601) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Cerebellum)
Antigen retrieval step
None
Permeabilization
Yes - Triton
Specification
Cerebellum
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Brandon Lee

Verified customer

Submitted Jun 20 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

Submitted Sep 16 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Cerebellum)
Specification
Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Apr 20 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cerebellum)
Specification
Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Apr 20 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Marmoset cerebellum)
Specification
Marmoset cerebellum
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Apr 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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