Anti-EAAT4 antibody (ab41650)
Key features and details
- Rabbit polyclonal to EAAT4
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-EAAT4 antibody
See all EAAT4 primary antibodies -
Description
Rabbit polyclonal to EAAT4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Dog, Human -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human EAAT4.
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Positive control
- This antibody gave a positive signal in Mouse Cerebellum tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 mg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 61 kDa).
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Notes |
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WB
Use a concentration of 1 mg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 61 kDa). |
Target
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Relevance
EAAT4 is expressed predominantly in the cerebellum (Purkinje cells) and weakly in the forebrain, where it transports L-glutamate, L-aspartate and D-aspartate, acting as a symport by cotransporting sodium. EAAT4 may function as a combined transporter and inhibitory glutamate receptor and also functions as an anion-selective ion channel. -
Cellular localization
Membrane; multi-pass membrane protein. -
Database links
- Entrez Gene: 403749 Dog
- Entrez Gene: 6511 Human
- Entrez Gene: 20513 Mouse
- Entrez Gene: 84012 Rat
- Omim: 600637 Human
- SwissProt: Q9N1R2 Dog
- SwissProt: P48664 Human
- SwissProt: O35544 Mouse
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Alternative names
- Excitatory amino acid transporter 4 antibody
- High affinity neuronal glutamate transporter antibody
- MGC33092 antibody
see all
Images
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Anti-EAAT4 antibody (ab41650) at 1 µg/ml + Cerebellum Mouse Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab41650 has been referenced in 3 publications.
- Kim YS et al. Neuroprotective effects of magnesium L-threonate in a hypoxic zebrafish model. BMC Neurosci 21:29 (2020). PubMed: 32590943
- Zhou L et al. MicroRNA-128 Protects Dopamine Neurons from Apoptosis and Upregulates the Expression of Excitatory Amino Acid Transporter 4 in Parkinson's Disease by Binding to AXIN1. Cell Physiol Biochem 51:2275-2289 (2018). PubMed: 30537735
- Ramachandran PS et al. Nonallele Specific Silencing of Ataxin-7 Improves Disease Phenotypes in a Mouse Model of SCA7. Mol Ther N/A:N/A (2014). PubMed: 24930601