Overview

  • Product name
  • Description
    Rabbit polyclonal to EAF2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    AGFSHLDRRERV

    , corresponding to amino acids 5-16 of Human EAF2.

  • Positive control
    • Rat and mouse liver for Western blot.

Properties

Applications

Our Abpromise guarantee covers the use of ab28357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notes
    IHC-P: Use at a concentration of 2.5 µg/ml.
    WB: Use at a concentration of 1.5 µg/ml. Predicted molecular weight: 29 kDa. This protein migrates to around 43kDa when a western blot is performed. We have found that the same happened in other literature as well. A paper published by Federico Simone shows that this protein can migrate to 43kDa (see references).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity). Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo.
    • Tissue specificity
      Expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, colon, adrenal, bone marrow, lymph node, spinal gland, stomach, thyroid, trachea, thymus, liver and leukocytes.
    • Sequence similarities
      Belongs to the EAF family.
    • Cellular localization
      Nucleus speckle. Colocalizes with ELL to nuclear speckles.
    • Information by UniProt
    • Database links
    • Alternative names
      • BM 040 antibody
      • BM040 antibody
      • EAF 2 antibody
      • EAF2 antibody
      • EAF2_HUMAN antibody
      • Ehrlich S II transcriptional activator factor antibody
      • ELL associated factor 2 antibody
      • ELL-associated factor 2 antibody
      • Festa antibody
      • Testosterone regulated apoptosis inducer and tumor suppressor antibody
      • Testosterone regulated apoptosis inducer and tumor suppressor protein antibody
      • Testosterone-regulated apoptosis inducer and tumor suppressor protein antibody
      • TRAITS antibody
      • U19 antibody
      • Uncharacterized bone marrow protein BM 040 antibody
      • Uncharacterized bone marrow protein BM040 antibody
      see all

    References

    ab28357 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T cell lysate)
    Loading amount
    30 µg
    Specification
    293T cell lysate
    Treatment
    Transfected with EAF2
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Dr. Laura Pascal

    Verified customer

    Submitted May 06 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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