Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) of Human testis tissue sections labelling EAN57 with ab121241 (1/20). Antigen retrieval was by HIER pH6. Immunohistochemical staining shows strong cytoplasmic and nuclear positivity in a subset of cells in seminiferus ducts.
Immunohistochemical staining of paraffin embedded Human duodenum tissue, using 1/35 ab121241, shows strong cytoplasmic positivity in enteroendocrine cells.
Western blot - Anti-EAN57 antibody (ab121241)
All lanes : Anti-EAN57 antibody (ab121241) at 1/250 dilution
Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2 : Negative control (vector only transfected HEK293T lysate)
Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)