• Product nameAnti-EAP30 antibody
    See all EAP30 primary antibodies
  • Description
    Rabbit polyclonal to EAP30
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Synthetic peptide, derived from a region between amino acids 1-55 of Human EAP30.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab22768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.


  • FunctionComponent of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4.
  • Sequence similaritiesBelongs to the SNF8 family.
  • Cellular localizationCytoplasm. Endosome membrane. Nucleus. Late endosome membrane. Recruited to the endosome membrane to participate in vesicle formation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dot3 antibody
    • ELL associated protein of 30 kDa antibody
    • ELL-associated protein of 30 kDa antibody
    • ESCRT-II complex subunit VPS22 antibody
    • hVps22 antibody
    • snf8 antibody
    • SNF8 ESCRTII complex subunit homolog (S cerevisiae) antibody
    • SNF8_HUMAN antibody
    • Vacuolar sorting protein SNF8 antibody
    • Vacuolar-sorting protein SNF8 antibody
    • VPS22 antibody
    see all

Anti-EAP30 antibody images

  • Anti-EAP30 antibody (ab22768) at 5 µg/ml + HepG2 Cell Lysate

    HRP conjugated anti-Rabbit IgG diluted in 1/50,000 - 100,000

    Performed under reducing conditions.

    Observed band size : 29 kDa (why is the actual band size different from the predicted?)
  • ab22768, at a 4.0-8.0 ug/ml dilution, staining human EAP30 in human stomach by immunohistochemistry, paraffin embedded tissue. Cells with positive label: epithelial cells of fundic gland (indicated with arrows) and surface mucous cells (indicated with arrow heads)
  • ab22768 staining EAP30 in Human heart tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections).
  • ab22768 staining EAP30 in Human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections).

References for Anti-EAP30 antibody (ab22768)

ab22768 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. You have followed the protocol recommended by our datasheet including the dilutions and blocking agent. Furthermore Eap30 expression has been ...

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