Overview

  • Product nameAnti-EAPP antibody
    See all EAPP primary antibodies
  • Description
    Rabbit polyclonal to EAPP
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to amino acids 32-142 of Human EAPP (Q56P03).

  • Positive control
    • Human adrenal gland tissue; Human cell line U251 MG.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab121154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

Target

  • FunctionMay play an important role in the fine-tuning of both major E2F1 activities, the regulation of the cell-cycle and the induction of apoptosis. Promotes S-phase entry, and inhibits p14(ARP) expression.
  • Tissue specificityUbiquitously expressed. Highest levels in heart, placenta, skeletal muscle and pancreas. Lower levels in brain, lung and kidney. In the brain, expressed in all regions with high levels in the cerebellum and cerebral cortex. Expressed in COS1 and transformed skin fibroblasts.
  • Developmental stageDuring the cell cycle, expression disappears during mitosis.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1810011O16Rik antibody
    • BM-036 antibody
    • BM036 antibody
    • C14orf11 antibody
    • E2F-associated phosphoprotein antibody
    • EAPP antibody
    • EAPP_HUMAN antibody
    • RGD1309624 antibody
    see all

Anti-EAPP antibody images

  • ab121154 at 1/200 dilution staining EAPP in Paraffin-emebedded Human Adrenal gland tissue by Immunohistochemistry.
    Note: strong nuclear positivity in cortical cells.
  • ab121154 at 4 µg/ml staining EAPP in PFA/Triton X-100 fixed U251 MG cells by Immunofluorescence (green).
    Note; positivity in nucleus but not nucleoli and golgi apparatus.

References for Anti-EAPP antibody (ab121154)

ab121154 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"