Overview

  • Product nameAnti-EB2 antibody [K52]
    See all EB2 primary antibodies
  • Description
    Rat monoclonal [K52] to EB2
  • SpecificityThis antibody is specific for EB2 proteins.
  • Tested applicationsSuitable for: WB, ELISA, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Hamster, Human
  • Immunogen

    Recombinant full length EB2 protein (Human)

  • Positive control
    • CHO cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab45767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 36 kDa.
ELISA 1/2000 - 1/5000.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionMay be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
  • Tissue specificityExpressed in different tumor cell lines. Up-regulated in activated B- and T-lymphocytes.
  • Sequence similaritiesBelongs to the MAPRE family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 EB1 C-terminal domain.
  • DomainComposed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
  • Cellular localizationCytoplasm > cytoskeleton. Associated with the microtubule network. Accumulates at the plus end of microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • APC binding protein EB1 antibody
    • APC binding protein EB2 antibody
    • APC-binding protein EB2 antibody
    • EB1 antibody
    • EB2 antibody
    • End binding protein 2 antibody
    • End-binding protein 2 antibody
    • H.sapiens mRNA for novel T cell activation protein antibody
    • MAPRE2 antibody
    • MARE2_HUMAN antibody
    • Microtubule associated protein RP/EB family member 2 antibody
    • Microtubule-associated protein RP/EB family member 2 antibody
    • RP1 antibody
    • T cell activation protein EB1 family antibody
    see all

Anti-EB2 antibody [K52] images

  • Immunofluorescence staining of EB2 in CHO cells transfected with EB2 gene expression constructs.

References for Anti-EB2 antibody [K52] (ab45767)

This product has been referenced in:
  • Asanov A  et al. A relay mechanism between EB1 and APC facilitate STIM1 puncta assembly at endoplasmic reticulum-plasma membrane junctions. Cell Calcium 54:246-56 (2013). Read more (PubMed: 23871111) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab45767.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"