Overview

  • Product nameAnti-EB3 antibody
    See all EB3 primary antibodies
  • Description
    Goat polyclonal to EB3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Cow, Dog
  • Immunogen

    Synthetic peptide:

    SGRLSNVAPPCILR

    , corresponding to internal sequence amino acids 172-185 of Human EB3 (NP_036458.2).

  • Positive control
    • Human Brain (Cerebellum and Amygdala) and Mouse Brain lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab99287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 32 kDa.

Target

  • FunctionMay be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
  • Tissue specificityPredominantly expressed in brain and muscle.
  • Sequence similaritiesBelongs to the MAPRE family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 EB1 C-terminal domain.
  • DomainComposed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
  • Cellular localizationCytoplasm > cytoskeleton. Associated with the microtubule network.
  • Information by UniProt
  • Database links
  • Alternative names
    • APC binding protein antibody
    • EB 3 antibody
    • EB1 protein family member 3 antibody
    • EB3 antibody
    • EBF 3 antibody
    • EBF3 antibody
    • EBF3 S antibody
    • End binding protein 3 antibody
    • End-binding protein 3 antibody
    • MAPRE 3 antibody
    • MAPRE3 antibody
    • MARE3_HUMAN antibody
    • Member 3 antibody
    • Microtubule Associated Protein antibody
    • Microtubule associated protein RP/EB family member 3 antibody
    • Microtubule-associated protein RP/EB family member 3 antibody
    • OTTHUMP00000200910 antibody
    • RP 3 antibody
    • RP/EB Family antibody
    • RP3 antibody
    see all

Anti-EB3 antibody images

  • Anti-EB3 antibody (ab99287) at 0.3 µg/ml + Mouse brain lysates at 35 µg

    Predicted band size : 32 kDa
  • Anti-EB3 antibody (ab99287) at 0.1 µg/ml + Human brain (amygdala) lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 32 kDa
    Primary incubation was 1 hour.

References for Anti-EB3 antibody (ab99287)

This product has been referenced in:
  • Oz S  et al. The NAP motif of activity-dependent neuroprotective protein (ADNP) regulates dendritic spines through microtubule end binding proteins. Mol Psychiatry 19:1115-24 (2014). Read more (PubMed: 25178163) »

See 1 Publication for this product

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