Recombinant Alexa Fluor® 647 Anti-EB3 antibody [EPR11421(B)] (ab203265)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR11421(B)] to EB3
- Suitable for: ICC/IF
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
Overview
-
Product name
Alexa Fluor® 647 Anti-EB3 antibody [EPR11421(B)]
See all EB3 primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EPR11421(B)] to EB3 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC/IF: Neuro-2a (differentiated) cells.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11421(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/50.
This product gave a positive signal in Neuro-2a (differentiated) cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
Notes |
---|
ICC/IF
1/50. This product gave a positive signal in Neuro-2a (differentiated) cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
Target
-
Function
May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. -
Tissue specificity
Predominantly expressed in brain and muscle. -
Sequence similarities
Belongs to the MAPRE family.
Contains 1 CH (calponin-homology) domain.
Contains 1 EB1 C-terminal domain. -
Domain
Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1. -
Cellular localization
Cytoplasm > cytoskeleton. Associated with the microtubule network. - Information by UniProt
-
Database links
- Entrez Gene: 22924 Human
- Entrez Gene: 100732 Mouse
- Entrez Gene: 298848 Rat
- Omim: 605788 Human
- SwissProt: Q9UPY8 Human
- SwissProt: Q6PER3 Mouse
- SwissProt: Q5XIT1 Rat
- Unigene: 515860 Human
see all -
Alternative names
- APC binding protein antibody
- EB 3 antibody
- EB1 protein family member 3 antibody
see all
Images
-
ab203265 staining EB3 in Neuro-2a (differentiated) cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203265 at a 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in Neuro-2a (differentiated) cells fixed with 100% methanol (5 min).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab203265 has not yet been referenced specifically in any publications.