Overview

  • Product name
    Anti-EB3 antibody [KT36]
    See all EB3 primary antibodies
  • Description
    Rat monoclonal [KT36] to EB3
  • Tested applications
    Suitable for: ELISA, ICC/IF, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human EB3 fused to a tag, purified from E. coli BL21 bacteria.

Properties

Applications

Our Abpromise guarantee covers the use of ab53360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF 1/100 - 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
Flow Cyt Use 1µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
  • Tissue specificity
    Predominantly expressed in brain and muscle.
  • Sequence similarities
    Belongs to the MAPRE family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 EB1 C-terminal domain.
  • Domain
    Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
  • Cellular localization
    Cytoplasm > cytoskeleton. Associated with the microtubule network.
  • Information by UniProt
  • Database links
  • Alternative names
    • APC binding protein antibody
    • EB 3 antibody
    • EB1 protein family member 3 antibody
    • EB3 antibody
    • EBF 3 antibody
    • EBF3 antibody
    • EBF3 S antibody
    • End binding protein 3 antibody
    • End-binding protein 3 antibody
    • MAPRE 3 antibody
    • MAPRE3 antibody
    • MARE3_HUMAN antibody
    • Member 3 antibody
    • Microtubule Associated Protein antibody
    • Microtubule associated protein RP/EB family member 3 antibody
    • Microtubule-associated protein RP/EB family member 3 antibody
    • OTTHUMP00000200910 antibody
    • RP 3 antibody
    • RP/EB Family antibody
    • RP3 antibody
    see all

Anti-EB3 antibody [KT36] images

  • Immunofluorescent staining for COS7 cells overexpressing EB3, using ab53360 at 1/100.
  • Immunofluorescent staining of cell mitosis using ab53360 crude culture supernatant diluted 1:10 (Please note that there was about 50-100 ug/ml antibody in the supernatants) COS7 Transformed African Green Monkey Kidney Fibroblast Cells.
  • Anti-EB3 antibody [KT36] (ab53360) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 32 kDa


    Exposure time : 8 minutes
  • Overlay histogram showing SH-SY5Y cells stained with ab53360 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53360, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-EB3 antibody [KT36] (ab53360)

This product has been referenced in:
  • Drum BM  et al. Oxidative stress decreases microtubule growth and stability in ventricular myocytes. J Mol Cell Cardiol 93:32-43 (2016). Read more (PubMed: 26902968) »
  • Adam MG  et al. SIAH ubiquitin ligases regulate breast cancer cell migration and invasion independent of the oxygen status. Cell Cycle 14:3734-47 (2015). WB . Read more (PubMed: 26654769) »

See all 4 Publications for this product

Product Wall

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain-fresh dissected hippocampus and lysed with p)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
25 µg
Specification
Brain-fresh dissected hippocampus and lysed with p
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Klaudio

Verified customer

Submitted May 15 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Cell (primary hippocampal neurons)
Specification
primary hippocampal neurons
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 25 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat-2 and cultured rat hippocampal neurons)
Specification
Rat-2 and cultured rat hippocampal neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X100
Username

Mrs. Marcelina Pieprzyk

Verified customer

Submitted Feb 05 2013

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Thank you for your reply.

Thats no problem. However, if you would like to participate in this scheme please do let us know prior to purchasing the antibody. I have generated the discount code as requested:

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Thank you for your reply.

The immunogen used to raise the anti-EB3 antibody [KT36] (ab53360) was of the recombinantly expressed full length human EB3 protein. This shares 99% homology with the rat and mouse protein. We would therefore predict...

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Thank you for contacting us and your interest in our products.

As yet, we have not tested the Anti-EB3 antibody [KT36] (ab53360) in any rodent species. However, this does not mean that the antibody would not work. As the immunogen used to rai...

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