• Product nameAnti-EB3 antibody [KT36]
    See all EB3 primary antibodies
  • Description
    Rat monoclonal [KT36] to EB3
  • Tested applicationsSuitable for: ELISA, ICC/IF, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human EB3 fused to a tag, purified from E. coli BL21 bacteria.



Our Abpromise guarantee covers the use of ab53360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF 1/100 - 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
Flow Cyt Use 1µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • FunctionMay be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
  • Tissue specificityPredominantly expressed in brain and muscle.
  • Sequence similaritiesBelongs to the MAPRE family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 EB1 C-terminal domain.
  • DomainComposed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
  • Cellular localizationCytoplasm > cytoskeleton. Associated with the microtubule network.
  • Information by UniProt
  • Database links
  • Alternative names
    • APC binding protein antibody
    • EB 3 antibody
    • EB1 protein family member 3 antibody
    • EB3 antibody
    • EBF 3 antibody
    • EBF3 antibody
    • EBF3 S antibody
    • End binding protein 3 antibody
    • End-binding protein 3 antibody
    • MAPRE 3 antibody
    • MAPRE3 antibody
    • MARE3_HUMAN antibody
    • Member 3 antibody
    • Microtubule Associated Protein antibody
    • Microtubule associated protein RP/EB family member 3 antibody
    • Microtubule-associated protein RP/EB family member 3 antibody
    • OTTHUMP00000200910 antibody
    • RP 3 antibody
    • RP/EB Family antibody
    • RP3 antibody
    see all

Anti-EB3 antibody [KT36] images

  • Immunofluorescent staining for COS7 cells overexpressing EB3, using ab53360 at 1/100.
  • Immunofluorescent staining of cell mitosis using ab53360 crude culture supernatant diluted 1:10 (Please note that there was about 50-100 ug/ml antibody in the supernatants) COS7 Transformed African Green Monkey Kidney Fibroblast Cells.
  • Anti-EB3 antibody [KT36] (ab53360) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 32 kDa

    Exposure time : 8 minutes
  • Overlay histogram showing SH-SY5Y cells stained with ab53360 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53360, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-EB3 antibody [KT36] (ab53360)

This product has been referenced in:
  • Patel DM  et al. Disease mutations in desmoplakin inhibit Cx43 membrane targeting mediated by desmoplakin-EB1 interactions. J Cell Biol 206:779-97 (2014). Read more (PubMed: 25225338) »
  • Asanov A  et al. A relay mechanism between EB1 and APC facilitate STIM1 puncta assembly at endoplasmic reticulum-plasma membrane junctions. Cell Calcium 54:246-56 (2013). Read more (PubMed: 23871111) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Brain-fresh dissected hippocampus and lysed with p)
Gel Running Conditions Reduced Denaturing (4-20%)
Loading amount 25 µg
Specification Brain-fresh dissected hippocampus and lysed with p
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C


Verified customer

Submitted May 15 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Cell (primary hippocampal neurons)
Specification primary hippocampal neurons
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 25 2013

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Rat-2 and cultured rat hippocampal neurons)
Specification Rat-2 and cultured rat hippocampal neurons
Fixative Paraformaldehyde
Permeabilization Yes - Triton X100

Mrs. Marcelina Pieprzyk

Verified customer

Submitted Feb 05 2013

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

Read More

Thank you for your reply.

Thats no problem. However, if you would like to participate in this scheme please do let us know prior to purchasing the antibody. I have generated the discount code as requested:

Discount code: xxxxxxx
Read More

Thank you for your reply.

The immunogen used to raise the anti-EB3 antibody [KT36] (ab53360) was of the recombinantly expressed full length human EB3 protein. This shares 99% homology with the rat and mouse protein. We would therefore predict...

Read More

Thank you for contacting us and your interest in our products.

As yet, we have not tested the Anti-EB3 antibody [KT36] (ab53360) in any rodent species. However, this does not mean that the antibody would not work. As the immunogen used to rai...

Read More