• Product name
  • Description
    Goat polyclonal to EBAG9
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog, Xenopus laevis
  • Immunogen

    Synthetic peptide:


    , corresponding to C terminal amino acids 195-206 of Human EBAG9

  • Positive control
    • A431 lysate.



Our Abpromise guarantee covers the use of ab52032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1:64,000.
    WB: Use at a concentration of 1.0 - 3.0 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa). A minor band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunising peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
    • Tissue specificity
      Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.
    • Domain
      The coiled coil domain is necessary for the homodimerization.
    • Cellular localization
      Golgi apparatus membrane. According to PubMed:10426319, it also exists as a soluble form which has the same biological activities. The existence of such soluble form is however uncertain.
    • Information by UniProt
    • Database links
    • Alternative names
      • BAG9 antibody
      • Cancer associated surface antigen antibody
      • Cancer associated surface antigen RCAS1 antibody
      • Cancer-associated surface antigen RCAS1 antibody
      • EB9 antibody
      • EBAG 9 antibody
      • EBAG9 antibody
      • Estrogen receptor binding fragment associated gene 9 protein antibody
      • Estrogen receptor binding site associated antigen 9 antibody
      • estrogen receptor binding site associated, antigen, 9 antibody
      • Estrogen receptor-binding fragment-associated gene 9 protein antibody
      • PDAF antibody
      • RCAS 1 antibody
      • RCAS1 antibody
      • RCAS1_HUMAN antibody
      • Receptor binding cancer antigen expressed on SiSo cells antibody
      • Receptor-binding cancer antigen expressed on SiSo cells antibody
      see all

    Anti-EBAG9 antibody images

    • All lanes : Anti-EBAG9 antibody (ab52032) at 0.2 µg/ml

      Lane 1 : A431 lysate
      Lane 2 : A431 lysate with Immunising peptide

      Lysates/proteins at 35 µg per lane.

      Predicted band size : 24 kDa
      Observed band size : 26 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-EBAG9 antibody (ab52032)

    This product has been referenced in:
    • Tskitishvili E  et al. The effect of tumor-associated protein RCAS1 gene silencing on blood pressure and urinary protein excretion in pregnant mouse: a pilot study. Am J Obstet Gynecol 203:364.e6-364.e12 (2010). WB ; Mouse . Read more (PubMed: 20579953) »

    See 1 Publication for this product

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