Anti-EBAG9 antibody - Carboxyterminal end (ab115202)

Overview

  • Product nameAnti-EBAG9 antibody - Carboxyterminal end
    See all EBAG9 primary antibodies
  • Description
    Goat polyclonal to EBAG9 - Carboxyterminal end
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Xenopus tropicalis
  • Immunogen

    Synthetic peptide:

    KEAQRLMKKEQN

    with a Cysteine residue linker, corresponding to C terminal amino acids 195-206 of Human EBAG9 (NP_004206.1).

  • Positive control
    • Human skeletal muscle tissue; A431 lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab115202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 3 µg/ml. Predicted molecular weight: 24 kDa.
ELISA 1/64000.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMay participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
  • Tissue specificityWidely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.
  • DomainThe coiled coil domain is necessary for the homodimerization.
  • Cellular localizationGolgi apparatus membrane. According to PubMed:10426319, it also exists as a soluble form which has the same biological activities. The existence of such soluble form is however uncertain.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAG9 antibody
    • Cancer associated surface antigen antibody
    • Cancer associated surface antigen RCAS1 antibody
    • Cancer-associated surface antigen RCAS1 antibody
    • EB9 antibody
    • EBAG 9 antibody
    • EBAG9 antibody
    • Estrogen receptor binding fragment associated gene 9 protein antibody
    • Estrogen receptor binding site associated antigen 9 antibody
    • estrogen receptor binding site associated, antigen, 9 antibody
    • Estrogen receptor-binding fragment-associated gene 9 protein antibody
    • PDAF antibody
    • RCAS 1 antibody
    • RCAS1 antibody
    • RCAS1_HUMAN antibody
    • Receptor binding cancer antigen expressed on SiSo cells antibody
    • Receptor-binding cancer antigen expressed on SiSo cells antibody
    see all

Anti-EBAG9 antibody - Carboxyterminal end images

  • ab115202 at 3.75 ug/ml, staining EBAG9 in Formalin-fixed, Paraffin-embedded Human skeletal muscle by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • All lanes : Anti-EBAG9 antibody - Carboxyterminal end (ab115202) at 0.2 µg/ml

    Lane 1 : A431 cell lysate (in RIPA buffer)
    Lane 2 : A431 cell lysate (in RIPA buffer) with immunizing peptide

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 24 kDa

References for Anti-EBAG9 antibody - Carboxyterminal end (ab115202)

ab115202 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"