The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.2 - 3 µg/ml. Predicted molecular weight: 24 kDa.
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.
The coiled coil domain is necessary for the homodimerization.
Golgi apparatus membrane. According to PubMed:10426319, it also exists as a soluble form which has the same biological activities. The existence of such soluble form is however uncertain.
ab115202 at 3.75 ug/ml, staining EBAG9 in Formalin-fixed, Paraffin-embedded Human skeletal muscle by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-EBAG9 antibody (ab115202)
All lanes : Anti-EBAG9 antibody - Carboxyterminal end (ab115202) at 0.2 µg/ml
Lane 1 : A431 cell lysate (in RIPA buffer) Lane 2 : A431 cell lysate (in RIPA buffer) with immunizing peptide