Overview

  • Product name
    Anti-EBF1 antibody [EPR4183]
    See all EBF1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4183] to EBF1
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human EBF1.

  • Positive control
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 64 kDa.

For unpurified use at 1/1000 - 1/10000.

  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function
      Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.
    • Sequence similarities
      Belongs to the COE family.
      Contains 1 IPT/TIG domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • COE1 antibody
      • COE1_HUMAN antibody
      • Collier, Olf and EBF transcription factor 1 antibody
      • Early B cell factor 1 antibody
      • Early B-cell factor antibody
      • EBF antibody
      • Ebf1 antibody
      • O/E 1 antibody
      • O/E-1 antibody
      • OE 1 antibody
      • OE-1 antibody
      • OE1 antibody
      • OLF1 antibody
      • olfactory neuronal transcription factor 1 antibody
      • Transcription factor COE1 antibody
      see all

    Anti-EBF1 antibody [EPR4183] images

    • Anti-EBF1 antibody [EPR4183] (ab108369) at 1/1000 dilution + Raji cell Lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 20 µg

      Predicted band size : 64 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST

    • All lanes : Anti-EBF1 antibody [EPR4183] (ab108369) at 1/1000 dilution (unpurified)

      Lane 1 : Raji cell lysate
      Lane 2 : Daudi cell lysate
      Lane 3 : 3T3-LI cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 64 kDa

    References for Anti-EBF1 antibody [EPR4183] (ab108369)

    This product has been referenced in:
    • Wang J  et al. RNA-binding protein PSPC1 promotes the differentiation-dependent nuclear export of adipocyte RNAs. J Clin Invest 127:987-1004 (2017). Read more (PubMed: 28192372) »
    • Woodcroft MW  et al. Retrovirus-Mediated Expression of E2A-PBX1 Blocks Lymphoid Fate but Permits Retention of Myeloid Potential in Early Hematopoietic Progenitors. PLoS One 10:e0130495 (2015). Read more (PubMed: 26098938) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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