Overview

  • Product name
  • Description
    Rabbit polyclonal to EBF2
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: IAEALYSVPR NPSQLPALSS SPAHSGMMGI NSYGSQLGVS ISESTQGNNQ, corresponding to C terminal amino acids 397-446 of Human EBF2

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab56048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • Function
    Transcription factor that, in osteoblasts, activates the decoy receptor for RANKL, TNFRSF11B, which in turn regulates osteoclast differentiation. Acts in synergy with the Wnt-responsive LEF1/CTNNB1 pathway. Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.
  • Sequence similarities
    Belongs to the COE family.
    Contains 1 IPT/TIG domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • COE 2 antibody
    • COE2 antibody
    • COE2_HUMAN antibody
    • Collier Olf and EBF 2 antibody
    • Early B cell factor 2 antibody
    • Early B-cell factor 2 antibody
    • EBF 2 antibody
    • EBF-2 antibody
    • Ebf2 antibody
    • FLJ11500 antibody
    • Metencephalon mesencephalnon olfactory transcription factor 1 antibody
    • O/E 3 antibody
    • O/E3 antibody
    • OE 3 antibody
    • OE3 antibody
    • OLF 1/EBF like 3 antibody
    • Transcription factor COE2 antibody
    see all

Images

  • Anti-EBF2 antibody (ab56048) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 63 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References

ab56048 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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