Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab77602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 65 kDa.
ELISA Use at an assay dependent dilution.

Target

Anti-EBF3 antibody images

  • Anti-EBF3 antibody (ab77602) at 5 µg/ml + immunogen (tagged 100 aa recombinant fragment) at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

    Predicted band size : 65 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-EBF3 antibody (ab77602) at 5 µg/ml + IMR-32 cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution

    Predicted band size : 65 kDa
    Observed band size : 65 kDa
    Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

References for Anti-EBF3 antibody (ab77602)

ab77602 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"