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Synthetic peptide: VLAMYDRQGQ PVEVERTAFI DFVEKDREPG TEKTNNGIHY RLRLVYNNGL, corresponding to a region within N terminal amino acids 72-121 of Human Ebf4
Our Abpromise guarantee covers the use of ab49182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 71 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay was 1:312500.
ab49182 has not yet been referenced specifically in any publications.