Overview

  • Product nameAnti-Ebf4 antibody
    See all Ebf4 primary antibodies
  • Description
    Rabbit polyclonal to Ebf4
  • SpecificityThis antibody regognizes Ebf4
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Dog, Human
  • Immunogen

    Synthetic peptide: VLAMYDRQGQ PVEVERTAFI DFVEKDREPG TEKTNNGIHY RLRLVYNNGL, corresponding to a region within N terminal amino acids 72-121 of Human Ebf4

  • Positive control
    • SP2/0 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 71 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay was 1:312500.

Target

Anti-Ebf4 antibody images

  • Anti-Ebf4 antibody (ab49182) at 2.5 µg/ml + SP2/0 lysate at 10 µg with 5% skim milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 71 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References for Anti-Ebf4 antibody (ab49182)

ab49182 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"