Overview

  • Product nameAnti-EBI3 antibody
    See all EBI3 primary antibodies
  • Description
    Rabbit polyclonal to EBI3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 200-230 of Mouse EBI3 (NP_056581.1).

  • Positive control
    • RAW cell lysate, Mouse brain tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab121041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 25 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionAssociates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 2 fibronectin type-III domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytokine receptor antibody
    • EBI3 antibody
    • EBV induced gene 3 protein antibody
    • EBV-induced gene 3 protein antibody
    • Epstein Barr virus induced 3 antibody
    • Epstein Barr virus induced gene 3 antibody
    • Epstein Barr virus induced gene 3 protein antibody
    • Epstein-Barr virus-induced gene 3 protein antibody
    • IL 27 subunit beta antibody
    • IL 27B antibody
    • IL-27 subunit beta antibody
    • IL-27B antibody
    • IL27 subunit antibody
    • IL27 subunit beta antibody
    • IL27B antibody
    • IL27B_HUMAN antibody
    • IL35 subunit antibody
    • interleukin 27 subunit beta antibody
    • Interleukin-27 subunit beta antibody
    see all

Anti-EBI3 antibody images

  • All lanes : Anti-EBI3 antibody (ab121041) at 0.5 µg/ml

    Lane 1 : RAW cell lysate
    Lane 2 : RAW cell lysate with immunizing peptide

    Lysates/proteins at 40 µg per lane.

    Secondary
    goat anti-rabbit Ig HRP
    Developed using the ECL technique

    Predicted band size : 25 kDa
  • Immunohistochemical analysis of EBI3 in formalin-fixed, paraffin-embedded Mouse brain tissue using an isotype control (top left) and ab121041 (bottom left, right) at 5 ug/ml.

References for Anti-EBI3 antibody (ab121041)

ab121041 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate 10mM, 0.05% Tween 20, pH 6.0
Permeabilization Yes - 0.05% Tween 20
Specification Skin
Blocking step BioGenex Power Block 10 minutes, then 5% Normal Donkey Serum 1 hour as blocking agent for 1 hour(s) and 10 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Formaldehyde
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Verified customer

Submitted Nov 19 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate 10mM, 0.05% Tween 20, pH 6.0
Permeabilization Yes - 0.05% Tween 20
Specification Skin
Blocking step BioGenex Power Block 10 minutes, then 5% Normal Donkey Serum 1 hour as blocking agent for 1 hour(s) and 10 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 10 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"