Overview

  • Product nameAnti-EBI3 antibody
    See all EBI3 primary antibodies
  • Description
    Rabbit polyclonal to EBI3
  • Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    16 amino acid synthetic peptide corresponding to an internal sequence of Human EBI3

  • Positive control
    • Human spleen.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab83896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 25 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • FunctionAssociates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 2 fibronectin type-III domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytokine receptor antibody
    • EBI3 antibody
    • EBV induced gene 3 protein antibody
    • EBV-induced gene 3 protein antibody
    • Epstein Barr virus induced 3 antibody
    • Epstein Barr virus induced gene 3 antibody
    • Epstein Barr virus induced gene 3 protein antibody
    • Epstein-Barr virus-induced gene 3 protein antibody
    • IL 27 subunit beta antibody
    • IL 27B antibody
    • IL-27 subunit beta antibody
    • IL-27B antibody
    • IL27 subunit antibody
    • IL27 subunit beta antibody
    • IL27B antibody
    • IL27B_HUMAN antibody
    • IL35 subunit antibody
    • interleukin 27 subunit beta antibody
    • Interleukin-27 subunit beta antibody
    see all

Anti-EBI3 antibody images

  • Anti-EBI3 antibody (ab83896) at 2 µg/ml + Rat spleen tissue lysate at 15 µg

    Predicted band size : 25 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ab83896 at 2.5µg/ml staining EBI3 in human spleen tissue.
  • Immunofluorescence of EBI3 in Human Spleen cells using ab83896 at 20 ug/ml.

References for Anti-EBI3 antibody (ab83896)

This product has been referenced in:
  • Al-Sammak F  et al. Gastric Epithelial Expression of IL-12 Cytokine Family in Helicobacter pylori Infection in Human: Is it Head or Tail of the Coin? PLoS One 8:e75192 (2013). IHC-P ; Human . Read more (PubMed: 24069393) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"