Anti-EBI3 antibody [B-G49] (Phycoerythrin) (ab84473)

Overview

  • Product name
    Anti-EBI3 antibody [B-G49] (Phycoerythrin)
    See all EBI3 primary antibodies
  • Description
    Mouse monoclonal [B-G49] to EBI3 (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    ab84473 is able to recognise EBI3, EBI3 associated as a heterodimer with the IL27 alpha subunit (aka p28) in IL27, and EBI3 associated as a heterodimer with the IL12 alpha subunit (aka p35) in IL35.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human EBI3.

  • Positive control
    • Dendritic cells.
  • General notes
    Stable at +4°C for 6 months after reconstitution.

Properties

  • Form
    Lyophilised:Reconstitute with 1ml of deionized water.
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 5% BSA, PBS
  • Concentration information loading...
  • Purity
    Ion Exchange Chromatography
  • Clonality
    Monoclonal
  • Clone number
    B-G49
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

Use 10µl for 106 cells or 100µl of whole blood.

Target

  • Function
    Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.
  • Sequence similarities
    Belongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 2 fibronectin type-III domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytokine receptor antibody
    • EBI3 antibody
    • EBV induced gene 3 protein antibody
    • EBV-induced gene 3 protein antibody
    • Epstein Barr virus induced 3 antibody
    • Epstein Barr virus induced gene 3 antibody
    • Epstein Barr virus induced gene 3 protein antibody
    • Epstein-Barr virus-induced gene 3 protein antibody
    • IL 27 subunit beta antibody
    • IL 27B antibody
    • IL-27 subunit beta antibody
    • IL-27B antibody
    • IL27 subunit antibody
    • IL27 subunit beta antibody
    • IL27B antibody
    • IL27B_HUMAN antibody
    • IL35 subunit antibody
    • interleukin 27 subunit beta antibody
    • Interleukin-27 subunit beta antibody
    see all

Images

  • Dendritic cells were activated with LPS (1µg/ml), IFN-gamma (10ng/ml) and TNF-alpha (100ng/ml) for 48 hours. A fraction of the dendritic cells were not activated to be used as negative control. 3.1 x 105 dendritic cells (activated = green line or resting cells = black line) were labelled with 10µl of ab84473.

References

ab84473 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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