Overview

  • Product nameAnti-EBI3 antibody [DNT27]
    See all EBI3 primary antibodies
  • Description
    Rat monoclonal [DNT27] to EBI3
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length Mouse EBI3

  • Positive control
    • Human Lyphoid-Dendritic Cells, Recombinant mouse EBI3/p28 (covalently linked IL-27) protein, mouse bone marrow-derived dendritic cells stimulated with LPS

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium azide
    Note: Aqueous buffer 99%
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityMonoclonal
  • Clone numberDNT27
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 25 kDa.
IHC-P Use a concentration of 10 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 24760014

Target

  • FunctionAssociates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 2 fibronectin type-III domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytokine receptor antibody
    • EBI3 antibody
    • EBV induced gene 3 protein antibody
    • EBV-induced gene 3 protein antibody
    • Epstein Barr virus induced 3 antibody
    • Epstein Barr virus induced gene 3 antibody
    • Epstein Barr virus induced gene 3 protein antibody
    • Epstein-Barr virus-induced gene 3 protein antibody
    • IL 27 subunit beta antibody
    • IL 27B antibody
    • IL-27 subunit beta antibody
    • IL-27B antibody
    • IL27 subunit antibody
    • IL27 subunit beta antibody
    • IL27B antibody
    • IL27B_HUMAN antibody
    • IL35 subunit antibody
    • interleukin 27 subunit beta antibody
    • Interleukin-27 subunit beta antibody
    see all

Anti-EBI3 antibody [DNT27] images

  • ab119500 at 10 µg/ml staining EBI3 in Human Lyphoid-Dendritic Cells by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
  • All lanes : Anti-EBI3 antibody [DNT27] (ab118500) at 5 µg/ml

    Lane 1 : Recombinant mouse EBI3/p28 (covalently linked IL-27) protein
    Lane 2 : Mouse bone marrow-derived dendritic cells stimulated with LPS
    Lane 3 : Untreated

    Secondary
    Horseradish peroxidase (HRP)-conjugated goat anti-rat IgG

    Predicted band size : 25 kDa

References for Anti-EBI3 antibody [DNT27] (ab118500)

This product has been referenced in:
  • Terayama H  et al. Contribution of IL-12/IL-35 common subunit p35 to maintaining the testicular immune privilege. PLoS One 9:e96120 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24760014) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Mouse spleen whole extract)
Loading amount 100 µg
Specification Mouse spleen whole extract
Gel Running Conditions Reduced Denaturing (10% Tris-glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver whole extract)
Loading amount 100 µg
Specification Liver whole extract
Gel Running Conditions Reduced Denaturing (14% Tris-glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 21 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"