Overview

  • Product nameAnti-EBI3 antibody [EPR5747]
    See all EBI3 primary antibodies
  • Description
    Rabbit monoclonal [EPR5747] to EBI3
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human EBI3 aa 200-300.

  • Positive control
    • Rat and Human spleen, Human tonsil,K562 and JAR cell lysates; permeabilized K562 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberEPR5747
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • FunctionAssociates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.
    • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 3 subfamily.
      Contains 2 fibronectin type-III domains.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • cytokine receptor antibody
      • EBI3 antibody
      • EBV induced gene 3 protein antibody
      • EBV-induced gene 3 protein antibody
      • Epstein Barr virus induced 3 antibody
      • Epstein Barr virus induced gene 3 antibody
      • Epstein Barr virus induced gene 3 protein antibody
      • Epstein-Barr virus-induced gene 3 protein antibody
      • IL 27 subunit beta antibody
      • IL 27B antibody
      • IL-27 subunit beta antibody
      • IL-27B antibody
      • IL27 subunit antibody
      • IL27 subunit beta antibody
      • IL27B antibody
      • IL27B_HUMAN antibody
      • IL35 subunit antibody
      • interleukin 27 subunit beta antibody
      • Interleukin-27 subunit beta antibody
      see all

    Anti-EBI3 antibody [EPR5747] images

    • All lanes : Anti-EBI3 antibody [EPR5747] (ab124694) at 1/5000 dilution (purified)

      Lane 1 : Mouse kidney tissue lysate
      Lane 2 : Mouse spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 25 kDa
      Observed band size : 25 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-EBI3 antibody [EPR5747] (ab124694) at 1/5000 dilution (purified)

      Lane 1 : Rat kidney tissue lysate
      Lane 2 : Rat spleen tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP anti-rabbit, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 25 kDa
      Observed band size : 25 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-EBI3 antibody [EPR5747] (ab124694) at 1/5000 dilution (purified) + Human tonsil tissue lysate at 10 µg

      Secondary
      HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 25 kDa
      Observed band size : 25 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-EBI3 antibody [EPR5747] (ab124694) at 1/5000 dilution (purified)

      Lane 1 : K562 cell lysate
      Lane 2 : JAR cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 25 kDa
      Observed band size : 25 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-EBI3 antibody [EPR5747] (ab124694) at 1/10000 dilution (unpurified)

      Lane 1 : Rat spleen tissue lysate
      Lane 2 : Human tonsil tissue lysate
      Lane 3 : K562 cell lysate
      Lane 4 : JAR cell lysate
      Lane 5 : Human spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 25 kDa

    References for Anti-EBI3 antibody [EPR5747] (ab124694)

    This product has been referenced in:
    • Wang WJ  et al. Regulation of the expression of Th17 cells and regulatory T cells by IL-27 in patients with unexplained early recurrent miscarriage. J Reprod Immunol 99:39-45 (2013). WB ; Human . Read more (PubMed: 23731956) »

    See 1 Publication for this product

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"