Overview

  • Product name
    Anti-EBNA 3A antibody
  • Description
    Sheep polyclonal to EBNA 3A
  • Specificity
    This antibody is specific for the Epstein Barr Virus Nuclear Antigen 3A.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Epstein Barr Virus
  • Immunogen

    Full length viral protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.08% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purification notes
    Purified by ammonium sulfate precipitation. This antibody is provided as a 0.2µm sterile filtered solution.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 22432076
WB Use a concentration of 0.25 - 1 µg/ml.

Target

  • Relevance
    EBNA3A is a latent viral nuclear protein expressed in Epstein Barr Virus (EBV) transformed lymphoblastic cell lines. It is also found in some immunoblastic lymphomas in vivo. This viral nuclear protein is essential for EBV mediated transformation of B lymphocytes. The EBNA3A functions as a transcriptional regulator though the target genes are currently unknown.
  • Alternative names
    • EBNA 3A antibody
    • EBV antibody
    • Epstein Barr virus antibody
    • HHV4 antibody
    • Nuclear antigen EBNA 3A antibody
    see all

References for Anti-EBNA 3A antibody (ab16126)

This product has been referenced in:
  • Bazot Q  et al. Epstein-Barr Virus Proteins EBNA3A and EBNA3C Together Induce Expression of the Oncogenic MicroRNA Cluster miR-221/miR-222 and Ablate Expression of Its Target p57KIP2. PLoS Pathog 11:e1005031 (2015). Read more (PubMed: 26153983) »
  • Quan L  et al. PD-1 Blockade Can Restore Functions of T-Cells in Epstein-Barr Virus-Positive Diffuse Large B-Cell Lymphoma In Vitro. PLoS One 10:e0136476 (2015). IHC-P ; Human . Read more (PubMed: 26361042) »

See all 4 Publications for this product

Product Wall

Thank you for your response.

As the datasheet indicates, the immunogen used to raise ab16126 was a full length viral protein. Since this is a polyclonal antibody, it recognizes multiple epitopes. For polyclonals, epitope mapping is not carrie...

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (Burkitt's lymphoma line)
Loading amount
50000 cells
Specification
Burkitt's lymphoma line
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted May 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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