Anti-Ebola virus antibody [FE37] (ab1926)

Overview

  • Product nameAnti-Ebola virus antibody [FE37]
    See all Ebola virus primary antibodies
  • Description
    Mouse monoclonal [FE37] to Ebola virus
  • SpecificityEbola virus, Zaire strain In Western blot they stain a single viral protein band corresponding to the envelope protein GP with a molecular mass of 74.5 kDa.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Immunogen

    Formalin inactivated Ebola virus (strain Zaire).

  • General notesConcentration varies from lot to lot and can be provided on request.


    Ebola virus is an enveloped, negative-stranded RNA virus. This virus belongs to the family Filoviridae and has a distinctive morphology similar only to that of Marburg virus, but apparently has no antigenic characteristics of Marburg virus. Its genome is a nonsegmented RNA strand. SDS-PAGE profiles demonstrate the presence of seven structural proteins. These are the major nucleoprotein NP (104 K), glycoprotein GP (125K), L protein (180 K), and four proteins: VP40 (40 K), ‘V’PX (35 K), VP30 (30 K) and VP24 (24 K). All the proteins are encoded by monocistronic mRNA transcripts complementary to virion RNA. GP is the major protein of the surface spikes and only this 125K viral protein is glycosylated.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurity tested by electrophoresis.
  • Primary antibody notesEbola virus is an enveloped, negative-stranded RNA virus. This virus belongs to the family Filoviridae and has a distinctive morphology similar only to that of Marburg virus, but apparently has no antigenic characteristics of Marburg virus. Its genome is a nonsegmented RNA strand. SDS-PAGE profiles demonstrate the presence of seven structural proteins. These are the major nucleoprotein NP (104 K), glycoprotein GP (125K), L protein (180 K), and four proteins: VP40 (40 K), ‘V’PX (35 K), VP30 (30 K) and VP24 (24 K). All the proteins are encoded by monocistronic mRNA transcripts complementary to virion RNA. GP is the major protein of the surface spikes and only this 125K viral protein is glycosylated.
  • ClonalityMonoclonal
  • Clone numberFE37
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

References for Anti-Ebola virus antibody [FE37] (ab1926)

ab1926 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample African Green Monkey Recombinant protein (Supernatant from VERO cells)
Gel Running Conditions Reduced Denaturing (4-15% Bio-Rad TGX gel)
Loading amount 10 µg
Specification Supernatant from VERO cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
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Submitted Aug 14 2015

Thank you for your enquiry. For the vial that you received, the concentration is 5.0 mg/ml. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"