Overview

  • Product nameAnti-EBP50 antibody
    See all EBP50 primary antibodies
  • Description
    Rabbit polyclonal to EBP50
  • SpecificityDetects EBP 50 from human T84 and Calu3 cells as well as recombinant human protein.
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
    Predicted to work with: Rabbit, Cow, Cynomolgus Monkey
  • Immunogen

    Recombinant fragment (His-tag) corresponding to Human EBP50. All His-tag specific antibody has been removed.
    Sequence:

    RSASSDTSEELNSQDSP

  • Positive control
    • T84 cell extract, mouse airway epithelium

Properties

Applications

Our Abpromise guarantee covers the use of ab3452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 15703401
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P Use a concentration of 10 µg/ml.

Target

  • FunctionScaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Interacts with MCC.
  • Tissue specificityDetected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
  • Involvement in diseaseDefects in SLC9A3R1 are the cause of hypophosphatemic nephrolithiasis/osteoporosis type 2 (NPHLOP2) [MIM:612287]. Hypophosphatemia results from idiopathic renal phosphate loss. It contributes to the pathogenesis of hypophosphatemic urolithiasis (formation of urinary calculi) as well to that of hypophosphatemic osteoporosis (bone demineralization).
  • Sequence similaritiesContains 2 PDZ (DHR) domains.
  • Post-translational
    modifications
    Phosphorylated on serine residues.
  • Cellular localizationCytoplasm. Apical cell membrane. Endomembrane system. Cell projection > filopodium. Cell projection > ruffle. Cell projection > microvillus. Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • EBP 50 antibody
    • EBP50 antibody
    • Ezrin radixin moesin binding phosphoprotein 50 antibody
    • Ezrin-radixin-moesin-binding phosphoprotein 50 antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE RF antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE-RF1 antibody
    • Na+/H+ exchange regulatory co factor antibody
    • NHERF 1 antibody
    • NHERF antibody
    • NHERF-1 antibody
    • NHERF1 antibody
    • NHRF1_HUMAN antibody
    • NPHLOP2 antibody
    • Regulatory cofactor of Na(+)/H(+) exchanger antibody
    • Slc9a3r1 antibody
    • Sodium hydrogen exchanger regulatory factor 1 antibody
    • Sodium-hydrogen exchanger regulatory factor 1 antibody
    • Sodium/hydrogen exchanger regulatory factor 1 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1 antibody
    • Solute carrier family 9 isoform 3 regulatory factor 1 antibody
    • Solute carrier family 9 isoform A3 regulatory factor 1 antibody
    • Solute carrier family 9 member 3 regulator 1 antibody
    see all

Anti-EBP50 antibody images

  • All lanes : Anti-EBP50 antibody (ab3452) at 1/1000 dilution

    Lane 1 : Whole cell lysate prepared from human HepG2 cells
    Lane 2 : Whole cell lysate prepared from human HepG2 cells

    Secondary
    HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa


    Exposure time : 40 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • All lanes : Anti-EBP50 antibody (ab3452) at 1/1000 dilution

    Lane 1 : Whole tissue lysate prepared from mouse liver
    Lane 2 : Whole tissue lysate prepared from mouse liver

    Secondary
    HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa


    Exposure time : 30 seconds

    This image is a courtsey of an anonymous abreview.

    See Abreview



  • Predicted band size : 50 kDa

    Western blot detection of recombinant Human EBP50 using ab3452.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse airway tissue sections labeling EBP50 with ab3452.

References for Anti-EBP50 antibody (ab3452)

This product has been referenced in:
  • Dudakovic A  et al. Histone deacetylase inhibition destabilizes the multi-potent state of uncommitted adipose-derived mesenchymal stromal cells. J Cell Physiol 230:52-62 (2015). WB ; Human . Read more (PubMed: 24912092) »
  • Kamiya K  et al. An unusually powerful mode of low-frequency sound interference due to defective hair bundles of the auditory outer hair cells. Proc Natl Acad Sci U S A 111:9307-12 (2014). ICC/IF ; Mouse . Read more (PubMed: 24920589) »

See all 20 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Sample Mouse Cell (hepatocytes)
Specification hepatocytes
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
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Submitted Jun 17 2014

Application Western blot
Sample Cow Tissue lysate - whole (Kidney tissue lysate)
Loading amount 20 µg
Specification Kidney tissue lysate
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
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Submitted Jan 10 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepG2)
Loading amount 20 µg
Specification HepG2
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Oct 30 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Loading amount 25 µg
Specification liver
Gel Running Conditions Reduced Denaturing (8)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

Submitted Oct 30 2008

Thank you for your patience in this matter. I have received a reply from the originator of ab3452 (EBP50 antibody). The epitope recognized by this product is amino acids 286-302 of the human EBP50 protein. This information has been added to the pr...

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I'm sorry to hear you are having problems with ab3452 and ab9526 and you had to re-send us your protocol details, I appreciate you taking the time to let us know those. I would like to recommend incubating the primary antibodies overnight at 4C and ...

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