• Product nameAnti-EBP50 antibody [EBP-10]
    See all EBP50 primary antibodies
  • Description
    Mouse monoclonal [EBP-10] to EBP50
  • SpecificityHuman ERM (ezrin/radixin/moesin)-binding phosphoprotein of 50 kDa (EBP50) / Na+/H+ exchange regulatory factor (NHERF)
  • Tested applicationsSuitable for: Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • RAJI human lymphoma cell lysate.



Our Abpromise guarantee covers the use of ab9526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Recommended dilution: 2µg/ml. Sample preparation: Lysis buffer with maltoside (1%). Application note: Non-reducing conditions with SDS-PAGE (10% separating gel).


  • FunctionScaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Interacts with MCC.
  • Tissue specificityDetected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
  • Involvement in diseaseDefects in SLC9A3R1 are the cause of hypophosphatemic nephrolithiasis/osteoporosis type 2 (NPHLOP2) [MIM:612287]. Hypophosphatemia results from idiopathic renal phosphate loss. It contributes to the pathogenesis of hypophosphatemic urolithiasis (formation of urinary calculi) as well to that of hypophosphatemic osteoporosis (bone demineralization).
  • Sequence similaritiesContains 2 PDZ (DHR) domains.
  • Post-translational
    Phosphorylated on serine residues.
  • Cellular localizationCytoplasm. Apical cell membrane. Endomembrane system. Cell projection > filopodium. Cell projection > ruffle. Cell projection > microvillus. Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • EBP 50 antibody
    • EBP50 antibody
    • Ezrin radixin moesin binding phosphoprotein 50 antibody
    • Ezrin-radixin-moesin-binding phosphoprotein 50 antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE RF antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE-RF1 antibody
    • Na+/H+ exchange regulatory co factor antibody
    • NHERF 1 antibody
    • NHERF antibody
    • NHERF-1 antibody
    • NHERF1 antibody
    • NHRF1_HUMAN antibody
    • NPHLOP2 antibody
    • Regulatory cofactor of Na(+)/H(+) exchanger antibody
    • Slc9a3r1 antibody
    • Sodium hydrogen exchanger regulatory factor 1 antibody
    • Sodium-hydrogen exchanger regulatory factor 1 antibody
    • Sodium/hydrogen exchanger regulatory factor 1 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1 antibody
    • Solute carrier family 9 isoform 3 regulatory factor 1 antibody
    • Solute carrier family 9 isoform A3 regulatory factor 1 antibody
    • Solute carrier family 9 member 3 regulator 1 antibody
    see all

Anti-EBP50 antibody [EBP-10] images

  • All lanes : Anti-EBP50 antibody [EBP-10] (ab9526) at 2 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HepG2 cells stained with ab9526 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9526, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-EBP50 antibody [EBP-10] (ab9526)

This product has been referenced in:
  • Wang B  et al. Ezrin-anchored PKA Coordinates Phosphorylation-dependent Disassembly of a NHERF1 Ternary Complex to Regulate Hormone-sensitive Phosphate Transport. J Biol Chem : (2012). Read more (PubMed: 22628548) »
  • Liu L  et al. Na+/H+ exchanger regulatory factor 1 (NHERF1) directly regulates osteogenesis. J Biol Chem 287:43312-21 (2012). WB ; Mouse . Read more (PubMed: 23109343) »

See all 6 Publications for this product

Product Wall

Thank you for your patience. I am sorry to hear that you are experiencing difficulties with this product ab9526 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the ...

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The epitope for this antibody has not been determined.

All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with dog has not yet been tested and the epitope for this antibody has not been fully characterised. Should you decide to go ahead...

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Try this antibody at 1:1000 for WB and adjust accordingly from the results obtained. For IP, please follow the guidelines in the IP protocol (linked on the right hand side of the Abcam homepage). The references are not accessible from the on-line datas...

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