Overview

  • Product name
    Anti-EBP50 antibody [EPR5562]
    See all EBP50 primary antibodies
  • Description
    Rabbit monoclonal [EPR5562] to EBP50
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • Positive control
    • HepG2, 293T, Jurkat, C6, PC-12 and MCF-7 cell lysates; Human kidney tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Interacts with MCC.
    • Tissue specificity
      Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
    • Involvement in disease
      Defects in SLC9A3R1 are the cause of hypophosphatemic nephrolithiasis/osteoporosis type 2 (NPHLOP2) [MIM:612287]. Hypophosphatemia results from idiopathic renal phosphate loss. It contributes to the pathogenesis of hypophosphatemic urolithiasis (formation of urinary calculi) as well to that of hypophosphatemic osteoporosis (bone demineralization).
    • Sequence similarities
      Contains 2 PDZ (DHR) domains.
    • Post-translational
      modifications
      Phosphorylated on serine residues.
    • Cellular localization
      Cytoplasm. Apical cell membrane. Endomembrane system. Cell projection > filopodium. Cell projection > ruffle. Cell projection > microvillus. Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • EBP 50 antibody
      • EBP50 antibody
      • Ezrin radixin moesin binding phosphoprotein 50 antibody
      • Ezrin-radixin-moesin-binding phosphoprotein 50 antibody
      • Na(+)/H(+) exchange regulatory cofactor NHE RF antibody
      • Na(+)/H(+) exchange regulatory cofactor NHE-RF1 antibody
      • Na+/H+ exchange regulatory co factor antibody
      • NHERF 1 antibody
      • NHERF antibody
      • NHERF-1 antibody
      • NHERF1 antibody
      • NHRF1_HUMAN antibody
      • NPHLOP2 antibody
      • Regulatory cofactor of Na(+)/H(+) exchanger antibody
      • Slc9a3r1 antibody
      • Sodium hydrogen exchanger regulatory factor 1 antibody
      • Sodium-hydrogen exchanger regulatory factor 1 antibody
      • Sodium/hydrogen exchanger regulatory factor 1 antibody
      • Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 antibody
      • Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1 antibody
      • Solute carrier family 9 isoform 3 regulatory factor 1 antibody
      • Solute carrier family 9 isoform A3 regulatory factor 1 antibody
      • Solute carrier family 9 member 3 regulator 1 antibody
      see all

    Images



    • Predicted band size : 39 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: EBP50 knockout HAP1 cell lysate (20 µg)
      Lane 3: Jurkat cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109430 observed at 48 kDa. Red - loading control, ab18058, observed at 124 kDa.

      ab109430 was shown to recognize EBP50 when EBP50 knockout samples were used, along with additional cross-reactive bands. Wild-type and EBP50 knockout samples were subjected to SDS-PAGE. ab109430 and ab18058 (loading control to Vinculin) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Immunohistochemical analysis of EBP50 in paraffin-embedded Human kidney tissue using ab109430 at 1/100 dilution.
    • All lanes : Anti-EBP50 antibody [EPR5562] (ab109430) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : C6 cell lysate
      Lane 5 : PC12 cell lysate
      Lane 6 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 39 kDa

    References

    This product has been referenced in:
    • Feng D  et al. Reduced EBP50 expression levels are correlated with unfavorable clinicopathological features of extrahepatic bile duct carcinoma and promote the proliferation and migration of QBC939 cells. Oncol Lett 13:2758-2764 (2017). IHC-P ; Human . Read more (PubMed: 28454463) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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