Anti-EBV Latent Membrane Protein 1 antibody [CS 1-4] (ab78113)

Overview

  • Product nameAnti-EBV Latent Membrane Protein 1 antibody [CS 1-4]
    See all EBV Latent Membrane Protein 1 primary antibodies
  • Description
    Mouse monoclonal [CS 1-4] to EBV Latent Membrane Protein 1
  • SpecificityThis antibody is specific for Epstein-Barr virus infected cells expressing the latent membrane protein (LMP). This is in fact four pooled antibodies which collectively detect LMP's encoded by each of 20 geographically distinct EBV isolates.
  • Tested applicationsSuitable for: WB, IP, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Epstein-Barr virus
  • Immunogen

    Fusion protein

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone numberCS 1-4
  • Myelomax63-Ag8.653
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • RelevanceEBV is a human herpesvirus that establishes a life-long persistence in the host. The virus infects the vast majority of the world's adult population and is well known for its association with a broad spectrum of benign and malignant diseases, including infectious mononucleosis, Burkitt's lymphoma, nasopharyngeal carcinoma, and is causally associated with lymphoid and epithelial malignancies, including post-transplant lymphoproliferative disorders, Hodgkin's disease, anaplastic nasopharyngeal carcinoma and gastric carcinomas. Latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is a transforming protein that affects multiple cell signalling pathways and contributes to EBV-associated oncogenesis. LMP1 can be expressed in some states of EBV latency, and significant induction of full-length LMP1 is also observed frequently during virus reactivation into the lytic cycle. LMP1 is critical for EBV-infected cell activation, adhesion and survival, and is usually expressed in the malignant cells.
  • Alternative names
    • BNLF1 antibody
    • EBV antibody
    • Epstein Barr virus antibody
    • HHV4 antibody
    • Human Herpesvirus 4 antibody
    • Latent membrane protein 1 antibody
    • LMP1 antibody
    see all

References for Anti-EBV Latent Membrane Protein 1 antibody [CS 1-4] (ab78113)

This product has been referenced in:
  • Martin KA  et al. Epstein-Barr Virus Oncoprotein LMP1 Mediates Epigenetic Changes in Host Gene Expression through PARP1. J Virol 90:8520-30 (2016). Read more (PubMed: 27440880) »
  • Campion EM  et al. Repression of the proapoptotic cellular BIK/NBK gene by Epstein-Barr virus antagonizes transforming growth factor ß1-induced B-cell apoptosis. J Virol 88:5001-13 (2014). Read more (PubMed: 24554662) »

See all 9 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (EBV transformed B cells)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 10 µg
Specification EBV transformed B cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jul 08 2015

Application Immunocytochemistry
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 20°C
Sample Mouse Cultured Cells (lymphocyte)
Specification lymphocyte
Permeabilization Yes - acetone
Fixative Methanol
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Submitted Jan 23 2015

Application Western blot
Loading amount 100 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-12% gel)
Sample Human Cell lysate - whole cell (PBMC)
Specification PBMC
Treatment EBV for 14days
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jan 23 2015

Application Immunocytochemistry
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Sample Mouse Cultured Cells (lymphoma)
Specification lymphoma
Permeabilization Yes - Acetone
Fixative Methanol
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Submitted Jan 23 2015

Vielen Dank für Ihre Antwort.

Leider muss ich Ihnen bestätigen, dass wir keinen Antikörper gegen die extrazelluläre Domäne in Produktion haben.

Ich werde diesen Vorschlag allerdings gerne weitergeben. Leider...

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Thank you for contacting Abcam. I have tried blasting the human EBV-LMP1 sequences against the rhesus monkey database and I cannot seem to get a hit for the rhesus EBV sequence (the only hit is for unnamed protein product, #21103, which has a 70% homol...

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Thank you for contacting us. The secondary antibody ab6785 (goat anti-mouse IgG, FITC-conjugated) is a suitable secondary for the three primary antibodies you asked about (ab78113, ab90543, and ab3280), as well as ab6276. I hope this inf...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"