Anti-EBV Latent Membrane Protein 1 antibody [CS 1-4] (ab78113)

Overview

  • Product name
    Anti-EBV Latent Membrane Protein 1 antibody [CS 1-4]
    See all EBV Latent Membrane Protein 1 primary antibodies
  • Description
    Mouse monoclonal [CS 1-4] to EBV Latent Membrane Protein 1
  • Specificity
    This antibody is specific for Epstein-Barr virus infected cells expressing the latent membrane protein (LMP). This is in fact four pooled antibodies which collectively detect LMP's encoded by each of 20 geographically distinct EBV isolates.
  • Tested applications
    Suitable for: WB, IP, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Epstein-Barr virus
  • Immunogen

    Fusion protein

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    CS 1-4
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    EBV is a human herpesvirus that establishes a life-long persistence in the host. The virus infects the vast majority of the world's adult population and is well known for its association with a broad spectrum of benign and malignant diseases, including infectious mononucleosis, Burkitt's lymphoma, nasopharyngeal carcinoma, and is causally associated with lymphoid and epithelial malignancies, including post-transplant lymphoproliferative disorders, Hodgkin's disease, anaplastic nasopharyngeal carcinoma and gastric carcinomas. Latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is a transforming protein that affects multiple cell signalling pathways and contributes to EBV-associated oncogenesis. LMP1 can be expressed in some states of EBV latency, and significant induction of full-length LMP1 is also observed frequently during virus reactivation into the lytic cycle. LMP1 is critical for EBV-infected cell activation, adhesion and survival, and is usually expressed in the malignant cells.
  • Database links
    • Alternative names
      • BNLF1 antibody
      • EBV antibody
      • Epstein Barr virus antibody
      • HHV4 antibody
      • Human Herpesvirus 4 antibody
      • Latent membrane protein 1 antibody
      • LMP1 antibody
      see all

    References

    This product has been referenced in:
    • Martin KA  et al. Epstein-Barr Virus Oncoprotein LMP1 Mediates Epigenetic Changes in Host Gene Expression through PARP1. J Virol 90:8520-30 (2016). Read more (PubMed: 27440880) »
    • Campion EM  et al. Repression of the proapoptotic cellular BIK/NBK gene by Epstein-Barr virus antagonizes transforming growth factor ß1-induced B-cell apoptosis. J Virol 88:5001-13 (2014). Read more (PubMed: 24554662) »

    See all 9 Publications for this product

    Customer reviews and Q&As

    Filter by Application

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    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (EBV transformed B cells)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    10 µg
    Specification
    EBV transformed B cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 08 2015

    Application
    Immunocytochemistry
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 20°C
    Sample
    Mouse Cultured Cells (lymphocyte)
    Specification
    lymphocyte
    Permeabilization
    Yes - acetone
    Fixative
    Methanol
    Username

    Abcam user community

    Verified customer

    Submitted Jan 23 2015

    Application
    Western blot
    Loading amount
    100 µg
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (4-12% gel)
    Sample
    Human Cell lysate - whole cell (PBMC)
    Specification
    PBMC
    Treatment
    EBV for 14days
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 23 2015

    Application
    Immunocytochemistry
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Sample
    Mouse Cultured Cells (lymphoma)
    Specification
    lymphoma
    Permeabilization
    Yes - Acetone
    Fixative
    Methanol
    Username

    Abcam user community

    Verified customer

    Submitted Jan 23 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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