Overview

  • Product name
  • Description
    Rabbit polyclonal to Ecat1
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: GTQRSVEVRE AGTQRSVEVQ EVGTQGSPVE VQEAGTQQSL QAANKSGTQR SPEAASKAVT QRFREDARDP VTRL, corresponding to amino acids of Human Ecat1.

  • Positive control
    • Human duodenum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab126339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/250.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Tissue specificity
    Expression appears to be maximal in germinal vesicle oocytes, it tails off through metaphase II oocytes and is undetectable following the completion of the oocyte to embryo transition.
  • Involvement in disease
    Defects in KHDC3L are the cause of hydatidiform mole recurrent type 2 (HYDM2) [MIM:614293]. A disorder characterized by excessive trophoblast development that produces a growing mass of tissue inside the uterus at the beginning of a pregnancy. It leads to abnormal pregnancies with no embryo, and cystic degeneration of the chorionic villi.
  • Sequence similarities
    Belongs to the KHDC1 family.
    Contains 1 KH domain.
  • Domain
    Contains an atypical KH domain with amino acid changes at critical sites, suggesting that it may not bind RNA.
  • Information by UniProt
  • Database links
  • Alternative names
    • C6orf221 antibody
    • Chromosome 6 open reading frame 221 antibody
    • ES cell-associated transcript 1 protein antibody
    • HYDM2 antibody
    • KHD3L_HUMAN antibody
    • KHDC3-like protein antibody
    • KHDC3L antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab126339 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1 :
    Lanes 2 - 3 : Anti-Ecat1 antibody (ab126339)

    Lane 1 : Marker
    Lane 2 : HEK293T cell lysate - vector only transfected
    Lane 3 : HEK293T cell lysate - overexpressing Ecat1 with a C-terminal myc-DDK tag (~3.1kDa)
  • ab126339 at 1/500 dilution staining Ecat1 in Paraffin-embedded Human duodenum tissue by Immunohistochemistry.

References

This product has been referenced in:
  • Virant-Klun I  et al. Identification of Maturation-Specific Proteins by Single-Cell Proteomics of Human Oocytes. Mol Cell Proteomics 15:2616-27 (2016). Human . Read more (PubMed: 27215607) »
  • Liu C  et al. ECAT1 is essential for human oocyte maturation and pre-implantation development of the resulting embryos. Sci Rep 6:38192 (2016). Read more (PubMed: 27917907) »

See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab126339.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up