Overview

  • Product name
  • Description
    Rabbit polyclonal to Ecat1
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Ecat1 aa 27-56 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q587J8

  • Positive control
    • HL60 cell lysate; Human testis tissue lysate; HL60 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170298 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 24 kDa.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificity
    Expression appears to be maximal in germinal vesicle oocytes, it tails off through metaphase II oocytes and is undetectable following the completion of the oocyte to embryo transition.
  • Involvement in disease
    Defects in KHDC3L are the cause of hydatidiform mole recurrent type 2 (HYDM2) [MIM:614293]. A disorder characterized by excessive trophoblast development that produces a growing mass of tissue inside the uterus at the beginning of a pregnancy. It leads to abnormal pregnancies with no embryo, and cystic degeneration of the chorionic villi.
  • Sequence similarities
    Belongs to the KHDC1 family.
    Contains 1 KH domain.
  • Domain
    Contains an atypical KH domain with amino acid changes at critical sites, suggesting that it may not bind RNA.
  • Information by UniProt
  • Database links
  • Alternative names
    • C6orf221 antibody
    • Chromosome 6 open reading frame 221 antibody
    • ES cell-associated transcript 1 protein antibody
    • HYDM2 antibody
    • KHD3L_HUMAN antibody
    • KHDC3-like protein antibody
    • KHDC3L antibody
    see all

Images

  • Anti-Ecat1 antibody (ab170298) at 1/100 dilution + HL60 cell lysate

    Predicted band size : 24 kDa
  • Immuhistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling Ecat1 with ab170298 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Flow cytometric analysis of HL60 cells labeling Ecat1 with ab170298 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram).

References

ab170298 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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