Anti-ECE1 antibody (ab71829)

Overview

  • Product name
  • Description
    Rabbit polyclonal to ECE1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Guinea pig, Cow, Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Human ECE1.

    (Peptide available as ab87264.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HUVEC MEF1 NIH 3T3

Properties

Applications

Our Abpromise guarantee covers the use of ab71829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 87 kDa).

Target

  • Relevance
    Function: Converts big endothelin-1 to endothelin-1. Tissue specificity: All isoforms are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A and C in liver, testis and small intestine; isoform B, C and D in endothelial cells and umbilical vein smooth muscle cells; isoforms C and D in saphenous vein cells, and isoform C in kidney. Disease: Hirschsprung disease cardiac defects and autonomic dysfunction Similarity: Belongs to the peptidase M13 family.
  • Cellular localization
    Cell Membrane. Single pass type II membrane protein.
  • Database links
  • Alternative names
    • ECE 1 antibody
    • ECE antibody
    • ECE-1 antibody
    • ECE1 antibody
    • ECE1_HUMAN antibody
    • Endothelin converting enzyme 1 antibody
    • Endothelin-converting enzyme 1 antibody
    see all

Images

  • All lanes : Anti-ECE1 antibody (ab71829) at 1 µg/ml

    Lane 1 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 87 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

References

ab71829 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (DLD-1 colon cancer cells)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
50 µg
Treatment
See comments
Specification
DLD-1 colon cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted Dec 09 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Permeabilization
Yes - Tween-20
Specification
pancreas tumor
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
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Verified customer

Submitted Aug 03 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
Yes - Tween-20
Specification
colon
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 29 2015


I am very pleased to hear you would like to accept our offer and test ab71829 in monkey]. This code will give you: 1 free PRIMARY ANTIBODY OR $337.00 OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for monk...

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