Anti-ECH1 antibody [3B7CF2AC8] (ab123450)

Overview

  • Product nameAnti-ECH1 antibody [3B7CF2AC8]
    See all ECH1 primary antibodies
  • Description
    Mouse monoclonal [3B7CF2AC8] to ECH1
  • Tested applicationsSuitable for: IHC-P, WB, IP, In-Cell ELISA, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Cow, Human
  • Immunogen

    Purified Human liver mitochondria.

  • Positive control
    • HDFn cells; Human, Rat and Cow heart homogenates; Human liver homogenate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab123450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa.
IP Use at an assay dependent concentration.
In-Cell ELISA Use a concentration of 1 µg/ml.
ICC Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • FunctionIsomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.
  • PathwayLipid metabolism; fatty acid beta-oxidation.
  • Sequence similaritiesBelongs to the enoyl-CoA hydratase/isomerase family.
  • Cellular localizationMitochondrion. Peroxisome.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4)-dienoyl-CoA isomerase antibody
    • 5)-Delta(2 antibody
    • Delta(3 5) Delta(2 4) dienoyl CoA isomerase antibody
    • Delta(3 5) Delta(2 4) dienoyl CoA isomerase mitochondrial antibody
    • Delta(3 antibody
    • Delta3 5 delta2 4 dienoyl CoA isomerase antibody
    • Dienoyl CoA isomerase antibody
    • ECH 1 antibody
    • Ech1 antibody
    • ECH1_HUMAN antibody
    • Enoyl Coenzyme A hydratase 1 antibody
    • Enoyl Coenzyme A hydratase 1 peroxisomal antibody
    • HPXEL antibody
    • mitochondrial antibody
    • MS1034 antibody
    • Peroxisomal enoyl CoA hydratase 1 antibody
    • Peroxisomal enoyl coenzyme A hydratase like protein antibody
    see all

Anti-ECH1 antibody [3B7CF2AC8] images

  • All lanes : Anti-ECH1 antibody [3B7CF2AC8] (ab123450) at 1 µg/ml

    Lane 1 : Human heart homogenate
    Lane 2 : Human liver homogenate
    Lane 3 : Rat heart homogenate
    Lane 4 : Cow heart homogenate

    Lysates/proteins at 10 µg/ml per lane.

    Secondary
    Goat-anti-Mouse HRP at 3000

    Predicted band size : 36 kDa
  • ab123450 at 1µg/ml staining ECH1 in 4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized HDFn cells by Immunocytochemistry followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). DAPI was used to stain the cell nuclei (blue).
  • ab123450 at 1µg/ml staining ECH1 in HDFn cells by Flow Cytometry (blue) or no primary antibody control (red).
  • IHC image of ECH1 staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab123450, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-ECH1 antibody [3B7CF2AC8] (ab123450)

ab123450 has not yet been referenced specifically in any publications.

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