Overview

  • Product nameAnti-ECHDC2 antibody
    See all ECHDC2 primary antibodies
  • Description
    Rabbit polyclonal to ECHDC2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A synthetic peptide within the range of amino acids 122-152 of human ECHDC2

  • Positive control
    • 721_B cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab75788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa.Can be blocked with ECHDC2 peptide (ab195585). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

  • RelevanceECHDC2 belongs to the enoyl-CoA hydratase/isomerase family. There are two named isforms.
  • Cellular localizationMitochondrion
  • Database links
  • Alternative names
    • 1300017C12Rik antibody
    • 2610009M20Rik antibody
    • D4Ertd765e antibody
    • Enoyl Coenzyme A hydratase domain containing 2 antibody
    • FLJ10948 antibody
    • RGD1308525 antibody
    • RP23-379K6.3 antibody
    see all

Anti-ECHDC2 antibody images

  • Anti-ECHDC2 antibody (ab75788) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa

References for Anti-ECHDC2 antibody (ab75788)

This product has been referenced in:
  • Du J  et al. Enoyl coenzyme a hydratase domain-containing 2, a potential novel regulator of myocardial ischemia injury. J Am Heart Assoc 2:e000233 (2013). Rat . Read more (PubMed: 24108764) »

See 1 Publication for this product

Product Wall

DISCOUNT CODE: ****** Expiration date: April 1, 2012 I am very pleased to hear you would like to accept our offer and test ab75788 in ICC/IF. This code will give you one free primary antibody before the expiration date. To redeem this offer, ple...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (heart, liver, lung brain)
Loading amount 30 µg
Specification heart, liver, lung brain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Yang Shi

Verified customer

Submitted Sep 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"