Overview

  • Product nameAnti-ECM29 antibody
    See all ECM29 primary antibodies
  • Description
    Rabbit polyclonal to ECM29
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human ECM29 aa 1821-1839 (C terminal).
    Sequence:

    RPELQEKAALLKKTLENLE

  • Positive control
    • WB: HeLa cell lysate. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab28666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB 1/200. Detects a band of approximately 205 kDa (predicted molecular weight: 205 kDa).

Target

  • RelevanceECM29 appears to be one of three proteins which associate significantly with proteasomes. ECM29 has been shown to enhance the stability of the proteasome. ECM29 deletion has been shown to produce 26S proteasomes which dissociated in the absence of ATP. It was thus suggested that ECM29 tethers the regulatory complex to the proteasome.
  • Cellular localizationEndoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Endosome. Cytoplasm › cytoskeleton › centrosome. Nucleus.
  • Database links
  • Alternative names
    • ECM29 homolog, proteasome accessory protein antibody
    • FLJ22036 antibody
    • KIAA0368 antibody
    • KIAA1962 antibody
    • Proteasome associated protein ECM29 homolog antibody
    • RP11-386D8.2 antibody
    see all

Anti-ECM29 antibody images

  • ab28666 stained HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28666 at 1µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-ECM29 antibody (ab28666) (dilution of 1/200) + HeLa cell extract

    Predicted band size : 205 kDa
    Observed band size : 205 kDa
    Detection of ECM29 in HeLa cells.
  • Immunofluorescent staining of Ecm29 in HeLa cells using ab28666 (1/200 dilution).

References for Anti-ECM29 antibody (ab28666)

ab28666 has not yet been referenced specifically in any publications.

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