Overview

  • Product nameAnti-ECM29 antibody
    See all ECM29 primary antibodies
  • Description
    Mouse polyclonal to ECM29
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to C terminal amino acids 1327 - 1845 of Human ECM29 (Q5VYK3-1)

  • Positive control
    • Placenta lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 240 kDa.

Target

  • RelevanceECM29 appears to be one of three proteins which associate significantly with proteasomes. ECM29 has been shown to enhance the stability of the proteasome. ECM29 deletion has been shown to produce 26S proteasomes which dissociated in the absence of ATP. It was thus suggested that ECM29 tethers the regulatory complex to the proteasome.
  • Cellular localizationEndoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Endosome. Cytoplasm › cytoskeleton › centrosome. Nucleus.
  • Database links
  • Alternative names
    • ECM29 homolog, proteasome accessory protein antibody
    • FLJ22036 antibody
    • KIAA0368 antibody
    • KIAA1962 antibody
    • Proteasome associated protein ECM29 homolog antibody
    • RP11-386D8.2 antibody
    see all

Anti-ECM29 antibody images

  • Anti-ECM29 antibody (ab89149) at 1/500 dilution + Human placenta lysate at 50 µg

    Predicted band size : 240 kDa
  • All lanes : Anti-ECM29 antibody (ab89149) at 1/500 dilution

    Lane 1 : Immunogen (519 aa protein fragment) - transfected 293T cell lysate
    Lane 2 : non transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 240 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)

References for Anti-ECM29 antibody (ab89149)

ab89149 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"