Overview

  • Product nameAnti-Eco1 antibody
    See all Eco1 primary antibodies
  • Description
    Mouse polyclonal to Eco1
  • SpecificityThis antibody is reactive against mammalian transfected lysate.
  • Tested applicationsSuitable for: WBmore details
  • Immunogen

    Recombinant fragment corresponding to Human Eco1 aa 669-840 (C terminal).
    Sequence:

    MVLPEDPKYALKKVDEIREMVDNDLGFQQAPLMCYSRTKTLLFISNDKKV VGCLIAEHIQWGYRVIEEKLPVIRSEEEKVRFERQKAWCCSTLPEPAICG ISRIWVFSMMRRKKIASRMIECLRSNFIYGSYLSKEEIAFSDPTPDGKLF ATQYCGTGQFLVYNFINGQNST


    Database link: Q5FWF5

  • Positive control
    • Transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab70442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 95 kDa).

The actual band size corresponds to the size of the immunizing protein fragment which was transfected into lysate. We do not yet have data to indicate that the antibody will work in endogenously expressed samples.

Target

  • FunctionAcetyltransferase required for the establishment of sister chromatid cohesion and couple the processes of cohesion and DNA replication to ensure that only sister chromatids become paired together. In contrast to the structural cohesins, the deposition and establishment factors are required only during S phase. Acts by mediating the acetylation of cohesin component SMC3.
  • Tissue specificityWidely expressed. Expressed in heart, brain, liver, placenta, lung, kidney and pancreas. Highly expressed in muscle.
  • Sequence similaritiesBelongs to the acetyltransferase family. GCN5 subfamily.
  • DomainThe N-terminal region seems to be responsible for the association with chromosomes, thus excluding any involvement of the Zn finger in this process.
  • Post-translational
    modifications
    Phosphorylated during mitosis. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Chromosome. Nuclear at interphase, associated with chromosomes during mitosis.
  • Information by UniProt
  • Alternative names
    • A930014I12Rik antibody
    • CTF antibody
    • CTF7 homolog 1 antibody
    • CTF7, S. cerevisiae, homolog of, 1 antibody
    • ECO1 antibody
    • ECO1 homolog 1 antibody
    • ECO1, S. cerevisiae, homolog of, 1 antibody
    • EFO1 antibody
    • EFO1p antibody
    • ESCO1 antibody
    • ESCO1_HUMAN antibody
    • ESO1 antibody
    • ESO1 homolog 1 antibody
    • ESO1, S. pombe, homolog of, 1 antibody
    • Establishment factor like protein 1 antibody
    • Establishment factor ortholog 1 antibody
    • Establishment factor-like protein 1 antibody
    • Establishment of cohesion 1 homolog 1 antibody
    • Establishment of cohesion 1, S. cerevisiae, homolog of antibody
    • establishment of sister chromatid cohesion N-acetyltransferase 1 antibody
    • hEFO1 antibody
    • HGNC:24645 antibody
    • KIAA1911 antibody
    • N acetyltransferase ESCO1 antibody
    • N-acetyltransferase ESCO1 antibody
    see all

Anti-Eco1 antibody images

  • All lanes : Anti-Eco1 antibody (ab70442) at 1/500 dilution

    Lane 1 : Eco1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg/ml per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 95 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)

    Actual band size corresponds to the size of the transfected immunizing protein fragment.

References for Anti-Eco1 antibody (ab70442)

ab70442 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"