Anti-Eco1 antibody - C-terminal (ab180100)

Overview

  • Product name
    Anti-Eco1 antibody - C-terminal
    See all Eco1 primary antibodies
  • Description
    Rabbit polyclonal to Eco1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Eco1 aa 661-687 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q5FWF5

  • Positive control
    • 293 cell line lysate and mouse spleen lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 95 kDa.

Target

  • Function
    Acetyltransferase required for the establishment of sister chromatid cohesion and couple the processes of cohesion and DNA replication to ensure that only sister chromatids become paired together. In contrast to the structural cohesins, the deposition and establishment factors are required only during S phase. Acts by mediating the acetylation of cohesin component SMC3.
  • Tissue specificity
    Widely expressed. Expressed in heart, brain, liver, placenta, lung, kidney and pancreas. Highly expressed in muscle.
  • Sequence similarities
    Belongs to the acetyltransferase family. GCN5 subfamily.
  • Domain
    The N-terminal region seems to be responsible for the association with chromosomes, thus excluding any involvement of the Zn finger in this process.
  • Post-translational
    modifications
    Phosphorylated during mitosis. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Chromosome. Nuclear at interphase, associated with chromosomes during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • A930014I12Rik antibody
    • CTF antibody
    • CTF7 homolog 1 antibody
    • CTF7, S. cerevisiae, homolog of, 1 antibody
    • ECO1 antibody
    • ECO1 homolog 1 antibody
    • ECO1, S. cerevisiae, homolog of, 1 antibody
    • EFO1 antibody
    • EFO1p antibody
    • ESCO1 antibody
    • ESCO1_HUMAN antibody
    • ESO1 antibody
    • ESO1 homolog 1 antibody
    • ESO1, S. pombe, homolog of, 1 antibody
    • Establishment factor like protein 1 antibody
    • Establishment factor ortholog 1 antibody
    • Establishment factor-like protein 1 antibody
    • Establishment of cohesion 1 homolog 1 antibody
    • Establishment of cohesion 1, S. cerevisiae, homolog of antibody
    • establishment of sister chromatid cohesion N-acetyltransferase 1 antibody
    • hEFO1 antibody
    • HGNC:24645 antibody
    • KIAA1911 antibody
    • N acetyltransferase ESCO1 antibody
    • N-acetyltransferase ESCO1 antibody
    see all

Images

  • Anti-Eco1 antibody - C-terminal (ab180100) at 1/1000 dilution + Mouse spleen tissue lysates at 35 µg

    Predicted band size: 95 kDa

  • Anti-Eco1 antibody - C-terminal (ab180100) at 1/1000 dilution + 293 cell line lysates at 35 µg

    Predicted band size: 95 kDa

References

ab180100 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% TritonX100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Jun 16 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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