Anti-E.coli O157:H7 Sakai rfbE antibody [27] (ab81131)


  • Product nameAnti-E.coli O157:H7 Sakai rfbE antibody [27]
  • Description
    Mouse monoclonal [27] to E.coli O157:H7 Sakai rfbE
  • Specificityab81131 recognizes recombinant Escherichia coli O157:H7 str. Sakai rfbE protein.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Recombinant fragment (Escherichia coli )


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Proclin
    Constituents: Tris HCl, Sodium citrate, pH 7.0
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number27
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab81131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: Use at an assay dependent dilution.
    WB: 1/1000. Predicted molecular weight: 42 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceE. coli O157:H7 is an enterohemorrhagic strain of the bacterium E. coli and a cause of foodborne illness. It is a gram negative rod shaped bacterium. The letter "O" (not a zero) in the name refers to the somatic antigen number, whereas the "H" refers to the flagella antigen. E. coli O157:H7 is markedly different from other pathogenic E. coli. In particular, the O157:H7 serotype is negative for invasiveness (sereny test), elaborates no colonization factors (CFA/I or CFA/II), doesn't produce heat stable or heat labile toxins and is non-hemolytic. In addition, E. coli O157:H7 is usually sorbitol negative whereas 93% of all E. coli ferment sorbitol. E. coli O157:H7 also lacks the ability to hydrolyze 4-methylumbelliferyl-beta-D-glucuronide (MUG) and does not grow at 45°C in the presence of 0.15% bile salts. Because of the latter characteristic this serotype cannot be isolated by using standard fecal coliform methods that include incubation at 45°C.
    • Database links
      • Alternative names
        • ECs2841 antibody
        • Escherichia coli O157:H7 str. Sakai antibody
        • Perosamine synthetase antibody

      Anti-E.coli O157:H7 Sakai rfbE antibody [27] images

      • All lanes : Anti-E.coli O157:H7 Sakai rfbE antibody [27] (ab81131) at 1/1000 dilution

        Lane 1 : Molecular weight markers
        Lane 2 : Recombinant Escherichia coli O157:H7 str. Sakai rfbE

        Predicted band size : 42 kDa
        Observed band size : 42 kDa
        Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

      References for Anti-E.coli O157:H7 Sakai rfbE antibody [27] (ab81131)

      ab81131 has not yet been referenced specifically in any publications.

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