Anti-E.coli O157:H7 Sakai rfbE antibody [27] (ab81131)

Overview

  • Product name
    Anti-E.coli O157:H7 Sakai rfbE antibody [27]
  • Description
    Mouse monoclonal [27] to E.coli O157:H7 Sakai rfbE
  • Specificity
    ab81131 recognizes recombinant Escherichia coli O157:H7 str. Sakai rfbE protein.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Recombinant fragment (Escherichia coli )

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Proclin
    Constituents: Tris HCl, Sodium citrate, pH 7.0
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    27
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: Use at an assay dependent dilution.
    WB: 1/1000. Predicted molecular weight: 42 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      E. coli O157:H7 is an enterohemorrhagic strain of the bacterium E. coli and a cause of foodborne illness. It is a gram negative rod shaped bacterium. The letter "O" (not a zero) in the name refers to the somatic antigen number, whereas the "H" refers to the flagella antigen. E. coli O157:H7 is markedly different from other pathogenic E. coli. In particular, the O157:H7 serotype is negative for invasiveness (sereny test), elaborates no colonization factors (CFA/I or CFA/II), doesn't produce heat stable or heat labile toxins and is non-hemolytic. In addition, E. coli O157:H7 is usually sorbitol negative whereas 93% of all E. coli ferment sorbitol. E. coli O157:H7 also lacks the ability to hydrolyze 4-methylumbelliferyl-beta-D-glucuronide (MUG) and does not grow at 45°C in the presence of 0.15% bile salts. Because of the latter characteristic this serotype cannot be isolated by using standard fecal coliform methods that include incubation at 45°C.
    • Database links
      • Alternative names
        • ECs2841 antibody
        • Escherichia coli O157:H7 str. Sakai antibody
        • Perosamine synthetase antibody

      Images

      • All lanes : Anti-E.coli O157:H7 Sakai rfbE antibody [27] (ab81131) at 1/1000 dilution

        Lane 1 : Molecular weight markers
        Lane 2 : Recombinant Escherichia coli O157:H7 str. Sakai rfbE


        Predicted band size : 42 kDa
        Observed band size : 42 kDa
        Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

      References

      ab81131 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      There are currently no Customer reviews or Questions for ab81131.
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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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