Overview

  • Product nameAnti-ECT2 antibody
    See all ECT2 primary antibodies
  • Description
    Goat polyclonal to ECT2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide: AENSVLTSTTGRT, corresponding to N terminal amino acids 2-14 of Human ECT2.

  • General notesGene Ontology terms - guanyl-nucleotide exchange factor activity; cell growth and/or maintenance; intracellular signaling cascade; intracellular.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab4863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: 1/500.
    WB: 1/200 - 1/500 (overnight incubation in blocking buffer). Block with 5% milk for 30 min to 1 hr.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.
    • Cellular localizationECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis
    • Database links
    • Alternative names
      • ECT 2 antibody
      • ECT2 protein antibody
      • Epithelial cell transforming sequence 2 antibody
      • Epithelial cell transforming sequence 2 oncogene antibody
      • Epithelial cell transforming sequence 2 oncogene protein antibody
      • FLJ10461 antibody
      • MGC138291 antibody
      see all

    Anti-ECT2 antibody images

    • This image was kindly supplied as part of the review submitted by Naslin Rasheed.MEF cells were incubated with ab4863 (1/500) for 2 hours at room temperature. The staining shows the protein is localised to the nucleus.

    References for Anti-ECT2 antibody (ab4863)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (colon)
    Specification colon
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M Tris Buffer (pH9.0)/ 0.01M citrate Buffer (pH6.0)
    Permeabilization No
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Ms. JIHYE HAN

    Verified customer

    Submitted Jan 29 2010

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Methanol
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 27°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 23 2009

    ab4863 is an investigative grade antibody which means it has only been characterised by ELISA against the immunogen. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your c...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"