Overview

  • Product name
  • Description
    Goat polyclonal to ECT2
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide: AENSVLTSTTGRT, corresponding to N terminal amino acids 2-14 of Human ECT2.

  • General notes
    Gene Ontology terms - guanyl-nucleotide exchange factor activity; cell growth and/or maintenance; intracellular signaling cascade; intracellular.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC/IF: 1/500.
    WB: 1/200 - 1/500 (overnight incubation in blocking buffer). Block with 5% milk for 30 min to 1 hr.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.
    • Cellular localization
      ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis
    • Database links
    • Alternative names
      • ECT 2 antibody
      • ECT2 protein antibody
      • Epithelial cell transforming sequence 2 antibody
      • Epithelial cell transforming sequence 2 oncogene antibody
      • Epithelial cell transforming sequence 2 oncogene protein antibody
      • FLJ10461 antibody
      • MGC138291 antibody
      see all

    Anti-ECT2 antibody images

    • This image was kindly supplied as part of the review submitted by Naslin Rasheed.MEF cells were incubated with ab4863 (1/500) for 2 hours at room temperature. The staining shows the protein is localised to the nucleus.

    References for Anti-ECT2 antibody (ab4863)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (colon)
    Specification
    colon
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.01M Tris Buffer (pH9.0)/ 0.01M citrate Buffer (pH6.0)
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Ms. JIHYE HAN

    Verified customer

    Submitted Jan 29 2010

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 27°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 23 2009

    ab4863 is an investigative grade antibody which means it has only been characterised by ELISA against the immunogen. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your c...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up