Overview

  • Product name
  • Description
    Rabbit polyclonal to ECT2
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 833 and 883 of human ECT2 (NP_060568.3)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance
      The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.
    • Cellular localization
      ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis
    • Database links
    • Alternative names
      • ECT 2 antibody
      • ECT2 protein antibody
      • Epithelial cell transforming sequence 2 antibody
      • Epithelial cell transforming sequence 2 oncogene antibody
      • Epithelial cell transforming sequence 2 oncogene protein antibody
      • FLJ10461 antibody
      • MGC138291 antibody
      see all

    Images

    • All lanes : Anti-ECT2 antibody (ab86604) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 100 kDa
      Observed band size : 100 kDa


      Exposure time : 3 minutes

    References

    ab86604 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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