Anti-ECT2 (phospho T790) antibody (ab51494)

Overview

  • Product nameAnti-ECT2 (phospho T790) antibody
    See all ECT2 primary antibodies
  • Description
    Rabbit polyclonal to ECT2 (phospho T790)
  • SpecificityReactivity occurs against human ECT2 protein at the pT790 residue.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Dog, Chimpanzee, Zebrafish
  • Immunogen

    Synthetic peptide:

    TKDMDSTLSRA

    , corresponding to amino acids 785-795 of Human ECT2

  • Positive control
    • HeLa cell lysates

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab51494 was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
ICC/IF
  • Application notesELISA: 1/10000 - 1/40000.
    ICC/IF: Use at an assay dependent dilution.
    WB: 1/1000 - 1/4000. Predicted molecular weight: 100 kDa.
    Less than 2% reactivity is observed against the non-phosphorylated form of
    the immunizing peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.
    • Cellular localizationECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis
    • Database links
    • Alternative names
      • ECT 2 antibody
      • ECT2 protein antibody
      • Epithelial cell transforming sequence 2 antibody
      • Epithelial cell transforming sequence 2 oncogene antibody
      • Epithelial cell transforming sequence 2 oncogene protein antibody
      • FLJ10461 antibody
      • MGC138291 antibody
      see all

    References for Anti-ECT2 (phospho T790) antibody (ab51494)

    ab51494 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa cells)
    Specification HeLa cells
    Fixative Methanol
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 27°C
    Username

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    Submitted Jul 29 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"