Anti-ECT2 (phospho T790) antibody (ab51494)

Overview

  • Product name
    Anti-ECT2 (phospho T790) antibody
    See all ECT2 primary antibodies
  • Description
    Rabbit polyclonal to ECT2 (phospho T790)
  • Specificity
    Reactivity occurs against human ECT2 protein at the pT790 residue.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Dog, Chimpanzee, Zebrafish
  • Immunogen

    Synthetic peptide:

    TKDMDSTLSRA

    , corresponding to amino acids 785-795 of Human ECT2

  • Positive control
    • HeLa cell lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab51494 was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
ICC/IF
  • Application notes
    ELISA: 1/10000 - 1/40000.
    ICC/IF: Use at an assay dependent dilution.
    WB: 1/1000 - 1/4000. Predicted molecular weight: 100 kDa.
    Less than 2% reactivity is observed against the non-phosphorylated form of
    the immunizing peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.
    • Cellular localization
      ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis
    • Database links
    • Alternative names
      • ECT 2 antibody
      • ECT2 protein antibody
      • Epithelial cell transforming sequence 2 antibody
      • Epithelial cell transforming sequence 2 oncogene antibody
      • Epithelial cell transforming sequence 2 oncogene protein antibody
      • FLJ10461 antibody
      • MGC138291 antibody
      see all

    References for Anti-ECT2 (phospho T790) antibody (ab51494)

    ab51494 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa cells)
    Specification
    HeLa cells
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 27°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 29 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up