Overview

  • Product name
    Anti-ECT2L antibody
  • Description
    Rabbit polyclonal to ECT2L
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: GQKAQSIGIF SDGDSREINL LQGYKIGVKN LLRPEVRDFW EKLGSYVATE EEGGHVDFFV PLGASEAGIE VLSQ, corresponding to amino acids 335-408 of Human ECT2L.

  • Positive control
    • Human rectum tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May act as a guanine nucleotide exchange factor (GEF).
  • Sequence similarities
    Contains 1 DH (DBL-homology) domain.
    Contains 1 F-box domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHGEF32 antibody
    • C6orf91 antibody
    • dJ509I19.2 antibody
    • dJ509I19.3 antibody
    • dJ509I19.5 antibody
    • ECT2 like antibody
    • ECT2L antibody
    • ECT2L_HUMAN antibody
    • Epithelial cell-transforming sequence 2 oncogene-like antibody
    • FBXO49 antibody
    • LFDH antibody
    • Lung-specific F-box and DH domain-containing protein antibody
    • Putative guanine nucleotide exchange factor LFDH antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122281 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122281, at 1/300 dilution, staining ECT2L in paraffin-embedded Human rectum tissue by Immunohistochemistry.

References

ab122281 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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