Anti-EDA2R antibody (ab37171)

Overview

  • Product nameAnti-EDA2R antibody
    See all EDA2R primary antibodies
  • Description
    Rabbit polyclonal to EDA2R
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length EDA2R (Human)

  • Positive control
    • HEK 293 (Human embryonic kidney cell line) whole cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab37171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).

Target

  • FunctionReceptor for EDA isoform A2, but not for EDA isoform A1. Mediates the activation of the NF-kappa-B and JNK pathways. Activation seems to be mediated by binding to TRAF3 and TRAF6.
  • Sequence similaritiesContains 3 TNFR-Cys repeats.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9430060M22Rik antibody
    • Ectodysplasin A2 isoform receptor antibody
    • Ectodysplasin A2 receptor antibody
    • EDA A2R antibody
    • EDA-A2 receptor antibody
    • EDA2R antibody
    • EDAA2R antibody
    • MGC124099 antibody
    • OTTMUSP00000019574 antibody
    • OTTMUSP00000019575 antibody
    • RP23-188N15.1 antibody
    • TNFRSF27 antibody
    • TNR27_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 27 antibody
    • Tumor necrosis factor receptor superfamily member XEDAR antibody
    • X linked ectodysplasin A2 receptor antibody
    • X linked ectodysplasin receptor antibody
    • X-linked ectodysplasin-A2 receptor antibody
    • XEDAR antibody
    see all

Anti-EDA2R antibody images

  • Lane 1 : Anti-EDA2R antibody (ab37171) at 0.5 µg/ml
    Lane 2 : Anti-EDA2R antibody (ab37171) at 1 µg/ml
    Lane 3 : Anti-EDA2R antibody (ab37171) at 2 µg/ml

    Lane 1 : Human 293 cell lysate
    Lane 2 : Human 293 cell lysate
    Lane 3 : Human 293 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 33 kDa
    Observed band size : 33 kDa
  • ab37171 at 10 µg/ml staining EDA2R in human skin tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

References for Anti-EDA2R antibody (ab37171)

ab37171 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 19 2013

Application Immunoprecipitation
Immuno-precipitation step Other - sepharose beads
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Total protein in input 100 µg
Username

Abcam user community

Verified customer

Submitted Jun 19 2013

Thank you for taking the time to complete our questionnaire and send these images. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
The details you have kindly provided will enable us to investigate thi...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
The details you have kindly provided will enable us to investigate this case ...

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Thank you very much for your interest in ab37171. To our knowledge, ab37171 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab37171 now, test it in mouse and submit feedback to us in the form of ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"