Overview

  • Product name
    Anti-EDARADD antibody
  • Description
    Rabbit polyclonal to EDARADD
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: PTISDLLNDQ DLLDVIRIKL DPCHPTVKNW RNFASKWGMS YDELCFLEQR PQSPTLEFLL RNSQRTVGQL MELCRLYHRA DVEKVLRRWV DEEWPKRERG DP, corresponding to amino acids 110-211 of Human EDARADD

  • Positive control
    • Human duodenum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

Target

  • Function
    Adapter protein that interacts with EDAR DEATH domain and couples the receptor to EDA signaling pathway during morphogenesis of ectodermal organs. Mediates the activation of NF-kappa-B.
  • Tissue specificity
    Detected in adult pancreas, placenta and fetal skin, and at lower levels in lung, thymus, prostate and testis.
  • Involvement in disease
    Defects in EDARADD are a cause of ectodermal dysplasia anhidrotic (EDA) [MIM:224900]; also known ectodermal dysplasia hypohidrotic autosomal recessive (HED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDA is characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands.
  • Sequence similarities
    Contains 1 death domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cr antibody
    • Crinkled homolog antibody
    • ectodysplasia A receptor associated death domain antibody
    • Ectodysplasin-A receptor-associated adapter protein antibody
    • ED3 antibody
    • EDA3 antibody
    • EDAD_HUMAN antibody
    • EDAR associated death domain antibody
    • EDAR-associated death domain protein antibody
    • EDARADD antibody
    • Protein crinkled homolog antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121581 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121581 at a 1/75 dilution, staining EDARADD in glandular cells of paraffin-embedded Human duodenum tissue by immunohistochemistry.

References

This product has been referenced in:
  • Vizoso M  et al. Aberrant DNA methylation in non-small cell lung cancer-associated fibroblasts. Carcinogenesis 36:1453-63 (2015). IHC . Read more (PubMed: 26449251) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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