Overview

  • Product name
  • Description
    Rabbit polyclonal to EDC3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 25-75 of Human EDC3 (NP_079359.2).

  • Positive control
    • 293T, HeLa, Jurkat, mouse TCMK-1 and mouse NIH3T3 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab168851 was affinity purified using an epitope specific to EDC3 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 56 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Binds single-stranded RNA. In the process of mRNA degradation, may play a role in mRNA decapping. May play a role in spermiogenesis and oogenesis.
  • Tissue specificity
    Expressed in theca and granulosa cells in ovary, and in spermatids of the meiotic division part II and apical membrane of Sertoli cells in testis (at protein level). Also expressed in brain and mammary gland.
  • Sequence similarities
    Belongs to the EDC3 family.
    Contains 1 DFDF domain.
    Contains 1 YjeF N-terminal domain.
  • Domain
    The DFDF domain is unstructured by itself. It assumes a helical fold upon interaction with DDX6.
  • Cellular localization
    Cytoplasm > P-body. Processing bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • EDC 3 antibody
    • edc3 antibody
    • EDC3_HUMAN antibody
    • enhancer of mRNA decapping 3 homolog (S. cerevisiae) antibody
    • Enhancer of mRNA decapping 3 homolog antibody
    • Enhancer of mRNA decapping 3, S. cerevisiae, homolog of antibody
    • Enhancer of mRNA decapping protein 3 antibody
    • Enhancer of mRNA-decapping protein 3 antibody
    • FLJ21128 antibody
    • FLJ31777 antibody
    • hYjeF_N2 antibody
    • hYjeF_N2-15q23 antibody
    • LSM 16 antibody
    • LSM16 antibody
    • LSM16 homolog (EDC3 S. cerevisiae) antibody
    • LSM16 homolog (EDC3, S. cerevisiae) antibody
    • LSM16 homolog antibody
    • YJDC antibody
    • YjeF domain containing antibody
    • YjeF domain containing protein 1 antibody
    • YjeF domain containing protein antibody
    • YjeF domain-containing protein 1 antibody
    • YjeF N-terminal domain-containing protein 2 antibody
    • YjeF_N2 antibody
    • YJEFN2 antibody
    see all

Images

  • All lanes : Anti-EDC3 antibody (ab168851) at 0.1 µg

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK-1 whole cell lysate
    Lane 5 : NIH3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 56 kDa


    Exposure time: 10 seconds
  • Detection of EDC3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168851 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with an exposure time of 10 seconds

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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